Anti-IL-13Rα1 (CD213a1) Monoclonal Antibody for Flow Cytometry - P78552

Core Product Specifications and Parameters

Biological Background: IL-13Rα1 Function and Localization

• Interleukin-13 receptor subunit alpha-1 (IL-13Rα1, also designated IL13RA1, CD213A1, CT19, NR4) is a low-affinity receptor for the cytokine interleukin-13 (IL-13).
• In complex with the IL4RA subunit, it forms a functional receptor for both IL-13 and IL-4, serving as an alternate accessory protein in IL-4 signaling but not substituting for IL2RG in enhancing IL-2 binding.
• The protein is a single-pass type I transmembrane receptor located on the cell membrane (UniProt: SUBCELLULAR LOCATION: Membrane).
• IL-13Rα1 is ubiquitously expressed, with highest levels in heart, liver, skeletal muscle, and ovary, and lower levels in brain, lung, and kidney; it is also found in B-cells, T-cells, and endothelial cells.
• UniProt annotations classify it as a glycoprotein with disulfide bonds, containing fibronectin type-III domains characteristic of the cytokine receptor family.
• Alternative splicing produces multiple isoforms, and the canonical protein has a predicted molecular weight of 48.8 kDa; the product datasheet notes observed bands at 31 kDa and 48 kDa, which may represent cleavage products or glycosylation variants.
• IL-13Rα1 is additionally recognized as a cancer/testis antigen (CT19), indicating potential relevance in tumor immunology.

Experimental Guidance and Technical Tips

• This antibody is supplied pre-conjugated with and validated for flow cytometry (FC); titrate to determine the optimal staining concentration for your specific experimental setup.
• The immunogen targets the extracellular domain (residues 29-343), so the antibody should recognize the native protein on live cells; use appropriate surface staining protocols and consider including a viability dye.
• Include proper controls such as isotype control, unstained cells, and fluorescence minus one (FMO) controls to accurately gate positive populations.
• Due to ubiquitous expression, expression levels may vary across cell types; validate in your target cell system and consider using known positive and negative controls (e.g., activated T cells or endothelial cells).
• For applications beyond FC (e.g., Western blot, IHC), in-house validation is advised; note that multiple bands around 31 and 48 kDa may be observed due to glycosylation or isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic developers and researchers, offering validated monoclonal antibodies like this anti-IL-13Rα1 clone alongside antibody pairs, bulk reagents, and rare target sourcing. Our integrated supply chain and technical consulting support oncology and immunology programs from early development through clinical application, reducing bottlenecks and accelerating your workflow. Contact us to explore customized sourcing solutions tailored to your project’s needs.