Anti-IL-13 Rabbit Monoclonal Antibody for FC, ELISA - P20109

Core Product Specifications and Parameters

Biological Background: IL-13 Function and Localization

• Interleukin-13 (IL-13) is a secreted cytokine that plays a critical role in allergic inflammation and immune responses to parasite infections. Related references: PMID:15361238
• IL-13 synergizes with IL2 to regulate interferon-gamma synthesis and stimulates the proliferation of B-cells, and the activation of eosinophils, basophils, and mast cells (By similarity).
• It downregulates proinflammatory Th1-driven responses, suppressing the synthesis of cytokines such as IL1, IL6, IL10, IL12, and TNF, partially through inhibition of NF-kappa-B (By similarity).
• IL-13 modulates the production of transmembrane and soluble forms of IL1RL1, thereby regulating IL33 activity. Related references: PMID:34789557
• Signaling is mediated via a receptor complex of IL4R and IL13RA1, which activates JAK1 and TYK2, leading to STAT6 phosphorylation. Related references: PMID:34795444, PMID:8871614
• IL13RA2 acts as a high-affinity decoy receptor that binds IL-13 and mediates its internalization and depletion. Related references: PMID:29305434
• The protein is secreted and undergoes glycosylation and disulfide bond formation.
• IL-13 induces inflammatory responses in esophageal epithelium, disrupting desmosome junctions by down-regulating DSG1. Related references: PMID:24220297

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to the mature mouse IL-13 sequence (amino acids 20-131), suggesting recognition of the secreted form.
• For intracellular flow cytometry, consider using cell stimulation with PMA/ionomycin or specific antigens, followed by fixation and permeabilization to enable detection.
• For ELISA, optimize coating and detection conditions using known IL-13 standards, and validate in the relevant biological matrix.
• Since IL-13 is secreted, sample preparation should include steps to capture the cytokine in cell supernatants or serum/plasma.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering comprehensive solutions from concept to clinic. For researchers and diagnostic manufacturers studying allergy and inflammation, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Additionally, we specialize in the procurement of raw materials for rare or challenging targets, ensuring seamless supply chain support.