Anti-IL-1 beta Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P01584

Core Product Specifications and Parameters

Biological Background: IL-1 beta Function and Localization

• IL-1β is a potent pro-inflammatory cytokine that induces prostaglandin synthesis, neutrophil influx, T-cell and B-cell activation, and fibroblast proliferation (PubMed:3920526). Related references: PMID:3920526
• It promotes Th17 differentiation and synergizes with IL-12 to induce IFN-γ synthesis from Th1 cells (PubMed:10653850). Related references: PMID:10653850
• Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL-6 (PubMed:12794819). Related references: PMID:12794819
• Upon pyroptosis, mature IL-1β is released through gasdermin-D (GSDMD) pores into the extracellular space (PubMed:33377178, PubMed:33883744). Related references: PMID:33377178 PMID:33883744
• Acts as a sensor for S.pyogenes infection in skin; cleaved and activated by the bacterial SpeB protease to contain bacterial growth (PubMed:28331908). Related references: PMID:28331908
• Localizes to the cytoplasm, and is secreted via lysosomes and extracellular exosomes.
• Primarily expressed in activated monocytes/macrophages at the protein level.
• Key biological keywords include cytokine, inflammatory response, pyrogen, and secreted.

Experimental Guidance and Technical Tips

• For WB, a band at ~31 kDa is expected; validate using recombinant IL-1β or stimulated macrophage lysates.
• For IF/ICC and IF-P, consider cell fixation, permeabilization, and secretion inhibitors (e.g., Brefeldin A) to accumulate intracellular signal.
• In ELISA applications, optimize antibody concentration and pairing; this polyclonal may serve as capture or detection reagent after appropriate validation.
• Cross-reactivity with mouse and rat IL-1β supports translational models, but verify in the specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined gateway to high-quality IVD raw materials, including validated antibodies for inflammation and cytokine targets like IL-1β. Our comprehensive portfolio encompasses monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—supporting your workflow from concept to clinic. We are committed to bridging supply gaps and accelerating your diagnostic development with reliable, one-stop access.