Anti-IL-1 alpha Rabbit Polyclonal Antibody for WB and ELISA - P01583

Core Product Specifications and Parameters

Biological Background: IL-1 alpha Function and Localization

• IL-1 alpha is a constitutively expressed cytokine present in nearly all resting non-hematopoietic cells, playing a critical role in inflammation and bridging innate and adaptive immunity.

Related references: PMID:26439902

• It binds to IL1R1 and IL1RAP to form the high-affinity interleukin-1 receptor complex.

Related references: PMID:17507369 PMID:2950091

• Signaling through this complex recruits MYD88, IRAK1, and IRAK4, leading to activation of NF-kappa-B and the MAPK pathways (p38, p42/p44, and JNK).

Related references: PMID:17507369 PMID:14687581

• Intracellular IL-1 alpha acts as an alarmin; upon cell death, it is passively released to signal tissue damage and induce inflammation.

Related references: PMID:15679580

• It directly senses DNA damage, acting as a genotoxic stress signal without loss of cell integrity.

Related references: PMID:26439902

• Subcellularly, it localizes to the nucleus, cytoplasm, and is secreted.

• Post-translational modifications include acetylation, phosphorylation, glycosylation, and myristoylation.

• Keywords: Inflammatory response, cytokine, mitogen, pyrogen, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 112-271 of human IL-1 alpha, which shares significant homology with mouse and rat proteins; consider sequence alignment when planning cross-species experiments.
• For WB, the predicted molecular weight is 31 kDa, but post-translational modifications may cause migration shifts; validate in the relevant sample system using appropriate controls.
• ELISA may be suitable for detecting secreted IL-1 alpha in cell culture supernatants or biological fluids; optimize coating antigen concentration and detection conditions for your specific matrix.
• Because IL-1 alpha is an alarmin released during cell necrosis, careful sample preparation (e.g., inclusion of protease inhibitors) may help minimize background in cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated polyclonal antibodies like this IL-1 alpha Rabbit pAb. Our portfolio supports inflammation and immune response research, offering reliable sourcing for recombinant proteins, bulk ancillary reagents, and custom antibody development services. From concept to clinic, we help accelerate your assay development with tailored procurement solutions and technical support.