Anti-IKZF3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UKT9

Core Product Specifications and Parameters

Biological Background: IKZF3 Function and Localization

• IKZF3 is the Ikaros family zinc finger protein 3, also known as Aiolos, and functions as a transcription factor.
• It plays a critical role in lymphocyte differentiation, particularly in regulating B-cell differentiation, proliferation, and maturation to an effector state.
• Involved in the control of apoptosis in T-cells through an IL-2-dependent mechanism, partly by regulating BCL2 expression.
• Localizes to both the nucleus and cytoplasm, consistent with its transcriptional regulatory role.
• Highest expression is observed in peripheral blood leukocytes, spleen, and thymus, underscoring its immune system-specific function.
• Contains zinc finger domains that mediate DNA binding and is subject to post-translational modifications such as phosphorylation and ubiquitination.
• Alternative splicing produces multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans residues 280-450 of human IKZF3, which may provide specific recognition of this region; consider sequence alignment to evaluate cross-reactivity with other Ikaros family members.
• This antibody has been tested for WB, IF/ICC, and ELISA applications in human and mouse samples; optimal dilutions and conditions should be determined empirically for each assay.
• For IF/ICC, appropriate fixation and permeabilization protocols should be validated, as IKZF3 localizes to both nuclear and cytoplasmic compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your immunology and diagnostic research with reliable raw materials like this IKZF3 antibody. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our sourcing network also covers rare targets, enabling comprehensive project support from concept to clinic.