Anti-IKZF3 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9UKT9

Core Product Specifications and Parameters

Biological Background: IKZF3 Function and Localization

• IKZF3 (Aiolos) is a zinc finger transcription factor belonging to the Ikaros family, which plays a critical role in lymphocyte development and differentiation.
• It regulates B-cell differentiation, proliferation, and maturation into effector states, and modulates apoptosis in T-cells by controlling BCL2 expression in an IL-2-dependent manner.
• The protein localizes to both the nucleus and cytoplasm, consistent with its transcriptional regulatory functions and potential shuttling mechanisms.
• IKZF3 is highly expressed in peripheral blood leukocytes, spleen, and thymus, reflecting its importance in immune cell biology.
• As a member of the Ikaros family, it contains zinc finger domains that mediate DNA binding and protein-protein interactions.
• Post-translational modifications such as phosphorylation and isopeptide bond formation (ubiquitination) regulate its activity, and alternative splicing generates multiple isoforms.
• Mutations in IKZF3 are associated with disease variants, underscoring its significance in immune disorders.

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide spanning residues 1-100 of human IKZF3; consider using this region as a positive control in peptide competition assays.
• For western blotting, the predicted protein weight is approximately 58 kDa; validate band specificity in relevant human or rat lysates (e.g., peripheral blood leukocytes).
• In immunofluorescence and immunohistochemistry, nuclear and/or cytoplasmic staining may be observed depending on cell type and activation state.
• When applying to ELISA, confirm coating conditions and antibody pair compatibility in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials for immune cell research, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-IKZF3 antibody supports studies on lymphocyte differentiation and related disorders. For specialized needs in rare target sourcing, CamelBio offers tailored procurement solutions to accelerate your diagnostic development.