Anti-IKZF3 Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q9UKT9

Core Product Specifications and Parameters

Biological Background: IKZF3 Function and Localization

• Zinc finger protein Aiolos (IKZF3) belongs to the Ikaros family of transcription factors and is involved in lymphocyte differentiation.
• IKZF3 plays an essential role in B-cell differentiation, proliferation, and maturation; it also regulates BCL2 expression and controls IL2-dependent apoptosis in T-cells.
• The protein localizes to both the nucleus and the cytoplasm, consistent with its dynamic role in transcription regulation.
• IKZF3 is highly expressed in peripheral blood leukocytes, spleen, and thymus, reflecting its lymphoid-specific function.
• Multiple isoforms are produced by alternative splicing.
• Key post-translational modifications include phosphorylation, ubiquitination (Ubl conjugation), and isopeptide bond formation.
• The protein contains C2H2-type zinc finger domains that mediate DNA binding and metal ion coordination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the C-terminal portion of human IKZF3; consider sequence alignment with mouse and rat to verify epitope conservation for cross-reactivity.
• For western blot, the predicted molecular weight is approximately 58 kDa; note that isoforms may appear as multiple bands.
• Validate the antibody in the relevant species and application before large-scale experiments, as cross-reactivity and performance may vary between samples.
• Optimal working dilutions and conditions should be determined empirically for each protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratory researchers, and institutions with streamlined access to premium IVD raw materials. Beyond this IKZF3 monoclonal antibody (suitable for lymphocyte and B-cell development research), our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio bridge your procurement needs from concept to clinical application with a single point of contact.