Anti-IKZF2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UKS7

Core Product Specifications and Parameters

Biological Background: IKZF2 (Helios) Function and Localization

• IKZF2, also known as Helios, is a zinc finger transcription factor belonging to the Ikaros family.
• It plays a critical role in stabilizing the noninflammatory phenotype of regulatory T cells (Tregs) (By similarity).
• Helios may mediate its effects through STAT5 signaling (By similarity).
• It binds to the IL2 promoter and represses IL2 transcription via epigenetic silencing, including deacetylation of core histones (PubMed:37316189). Related references: PMID:37316189
• In Tregs, Helios is required for the recruitment of FOXP3 to the IL2 promoter (By similarity).
• IKZF2 is essential for the maturation of outer hair cells in the cochlea, and thus for normal hearing (By similarity).
• Subcellularly, IKZF2 localizes to the nucleus.
• It is expressed in various T cell types, including developing thymocytes, activated T cells, and mucosal-associated invariant T (MAIT) cells (PubMed:34826260). Related references: PMID:34826260
• IKZF2 is subject to post-translational modifications, including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 300-440 of human IKZF2, which may confer cross-species reactivity; verify sequence conservation if using non-human samples.
• For Western blotting, the expected molecular weight is approximately 60 kDa. Consider using lysates from Treg cells or IKZF2-expressing cell lines as positive controls.
• In ELISA, titrate the antibody against the immunogen or target protein to determine the optimal working dilution.
• As a polyclonal antibody, it may recognize multiple epitopes; validate specificity in your sample system (e.g., human, mouse, rat) with appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. For studies on IKZF2 (Helios) in regulatory T cell biology, IL-2 regulation, and hearing research, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing services streamline access to rare-target reagents, ensuring seamless progression from concept to clinical application.