Anti-IKKβ Rabbit Polyclonal Antibody for WB, ELISA - O14920

Core Product Specifications and Parameters

Biological Background: IKKβ Function and Localization

• IKKβ, also known as IKK2 or IKBKB, is a serine/threonine kinase and the catalytic subunit of the IκB kinase (IKK) complex, essential for canonical NF-kappa-B signaling. Related references: PMID:9346484
• It phosphorylates inhibitors of NF-kappa-B (IκBs) on critical serine residues, triggering their ubiquitination and proteasomal degradation, thereby freeing NF-kappa-B to translocate to the nucleus and activate transcription of immune, proliferative, and anti-apoptotic genes. Related references: PMID:20434986, PMID:20797629, PMID:21138416
• Beyond IκBs, IKKβ phosphorylates multiple signaling components, including NEMO, RELA, NFKB1, TBK1, and IKBKE, as well as substrates such as FOXO3, NAA10, NCOA3, BCL10, IRS1, RIPK1, and IRF5, fine-tuning immune responses and cell death pathways. Related references: PMID:11297557, PMID:14673179, PMID:20410276, PMID:15084260, PMID:17213322, PMID:19716809, PMID:25326418
• Following LPS-induced TLR4 endocytosis, IKKβ phosphorylates STAT1 at Thr-749, promoting ARID5A expression and IL-6 stabilization, as well as IL-12B transcription, linking innate inflammation to adaptive immunity. Related references: PMID:38621137, PMID:32209697
• IKKβ is located in the cytoplasm, nucleus, and membrane rafts, consistent with its dynamic roles in signal transduction.
• It is highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, and peripheral blood, reflecting its broad regulatory functions.
• Keywords highlight its involvement in phosphorylation, acetylation, ubiquitination, host-virus interactions, and disease variants, with a predicted molecular weight of ~87 kDa (though experimental detection ranges from 29–87 kDa due to isoforms/cleavage).

Experimental Guidance and Technical Tips

• For Western blot, a band corresponding to full-length IKKβ is expected near 87 kDa; however, multiple bands between 29–87 kDa may be observed due to alternative splicing, degradation, or post-translational modifications. Validate using positive controls (e.g., TNFα-stimulated HeLa or Jurkat cells) to confirm specificity.
• In ELISA, the antibody may be used as capture or detection reagent; consider pairing with a validated monoclonal antibody to IKKβ for sandwich ELISA development. Titration experiments are recommended to determine optimal working concentrations.
• Because the immunogen spans amino acids 400–669, the antibody targets the C-terminal half of the protein, which may not recognize N-terminal truncations or certain disease-associated mutants; cross-reactivity with close family members (IKKα) should be assessed experimentally using recombinant proteins or knockout lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your search for IVD raw materials by offering this anti-IKKβ rabbit polyclonal antibody as part of a comprehensive catalog supporting NF-kappa-B pathway research and diagnostic assay development. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—bridging the gap between concept and clinic.