Anti-IKKβ Rabbit pAb for WB, IF/ICC, ELISA - O14920

Core Product Specifications and Parameters

Biological Background: IKKβ Function and Localization

• IKKβ is a serine/threonine-protein kinase that serves as a critical catalytic subunit of the canonical IKK complex, essential for activating NF-κB signaling downstream of pro-inflammatory cytokines, pattern-recognition receptors, and genotoxic stress. Related references: PMID:9346484
• It phosphorylates inhibitor of κB (IκB) proteins on conserved serine residues, triggering their K48-linked polyubiquitination and proteasomal degradation; this liberates NF-κB dimers (e.g., p65/p50) for nuclear translocation and transcription of immune, survival, and inflammatory genes. Related references: PMID:20434986, PMID:20797629, PMID:21138416
• Beyond IκB substrates, IKKβ phosphorylates multiple signaling partners—including NEMO/IKBKG, RELA, and the IKK-related kinases TBK1 and IKBKE—to provide both feedforward activation and negative regulatory feedback within the NF-κB network. Related references: PMID:11297557, PMID:14673179, PMID:20410276
• The kinase also targets non-canonical substrates such as FOXO3 (mediating TNF-induced inactivation of this pro-apoptotic transcription factor) and STAT1 (phosphorylated at Thr-749 to promote ARID5A-mediated stabilization of IL-6 and activation of IL-12B), thereby integrating NF-κB with apoptotic and innate immune pathways. Related references: PMID:15084260, PMID:32209697, PMID:38621137
• Subcellular fractionation and immunofluorescence studies localize IKKβ predominantly to the cytoplasm, with signal-dependent translocation to the nucleus and recruitment to membrane rafts upon certain stimuli.
• Tissue expression profiling reveals high IKKβ levels in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, and peripheral blood, consistent with its role in ubiquitous inflammatory control.
• Post-translational modifications—including acetylation, hydroxylation, S-nitrosylation, and isopeptide (ubiquitin-like) conjugation—fine-tune IKKβ stability, activity, and interaction with regulatory partners.
• Clinically, loss-of-function mutations in IKBKB are linked to severe combined immunodeficiency (SCID) and other immune dysregulation syndromes, underscoring its non-redundant role in immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen spans residues 400–669 in the C-terminal half of human IKKβ. This region lies distal to the kinase domain and may detect multiple splice variants or proteolytic fragments, consistent with the reported 29–87 kDa range observed in Western blot. Consider using protease-inhibitor cocktails during sample preparation to preserve full-length species.
• For Western blotting, validate specificity by siRNA knockdown or CRISPR-Cas9 knockout in the relevant cell system, and compare with the predicted full-length molecular weight of ~87 kDa. For IF/ICC, anticipate both cytoplasmic and inducible nuclear staining; co-stain with a nuclear marker and verify with appropriate negative controls.
• In ELISA applications, employ recombinant IKKβ protein or peptide immunogen as a positive capture control, and titrate the antibody using a standard indirect protocol to optimize signal-to-noise.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio equips diagnostic manufacturers, laboratories, and research institutes with a complete portfolio of IVD raw materials, technical services, and regulatory consulting—supporting projects from concept to commercialization. Our catalog features validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to accelerate your assay development.
• The IKKβ Rabbit pAb (O14920) exemplifies our commitment to delivering high-quality reagents for immunoassay and inflammation research. Leverage CamelBio as your single procurement partner for all IKKβ-related raw materials, including matched detection antibodies, recombinant proteins, and specialty buffers required for reproducible diagnostic workflows.