Anti-IKKγ Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9Y6K9

Core Product Specifications and Parameters

Biological Background: IKKγ Function and Localization

• IKKγ, also known as NEMO or IKBKG, is the regulatory subunit of the canonical IκB kinase (IKK) complex, essential for NF-κB signaling. It acts as a scaffold coordinating the kinase subunits IKKα and IKKβ.
• The core function of IKKγ is to facilitate the phosphorylation and subsequent ubiquitin-dependent degradation of NF-κB inhibitors (IκBs), thereby releasing NF-κB for nuclear translocation and target gene activation (PubMed:14695475, PubMed:20724660, PubMed:21518757). Related references: PMID:14695475 PMID:20724660 PMID:21518757
• It specifically recognizes and binds polyubiquitin chains, exhibiting high affinity for linear (M1-linked) and Lys-63-linked ubiquitin. This ubiquitin-sensing ability is critical for IKK activation downstream of TNF, IL-1, and Toll-like receptors (PubMed:16547522, PubMed:18287044, PubMed:19033441). Related references: PMID:16547522 PMID:18287044 PMID:19033441
• IKKγ shuttles between the cytoplasm and the nucleus, contributing to DNA damage responses and transcriptional regulation.
• The protein is involved in antiviral innate immunity: it is essential for IRF3 activation following viral infection (PubMed:19854139) and mediates TLR3/IFIH1 pathways via Lys-27-linked polyubiquitination (PubMed:20724660). Related references: PMID:19854139 PMID:20724660
• IKKγ is broadly expressed in human tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Mutations in the IKBKG gene are linked to several genetic disorders such as incontinentia pigmenti, anhidrotic ectodermal dysplasia with immunodeficiency, and osteopetrosis.
• The protein contains a coiled-coil domain and a C-terminal zinc finger that mediates protein-protein interactions and ubiquitin binding.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering nearly full-length human IKKγ (aa 1-419). As a rabbit polyclonal antibody, it may recognize multiple epitopes and detect various isoforms or post-translationally modified forms of the target.
• For Western blot, multiple bands may be observed (predicted molecular weights of 37, 48, and 56 kDa), possibly representing splice variants or modified protein species. Validate the detection and relative band intensity using appropriate positive and negative control lysates from human or mouse cell lines.
• In immunoprecipitation, pre-clearing steps and appropriate lysis buffer conditions should be optimized, as IKKγ exists in a large multi-protein complex.
• For ELISA applications, consider using a matched antibody pair for capture and detection, and verify sensitivity with recombinant IKKγ or known positive samples.
• Owing to the high cross-reactivity with mouse, this antibody is suitable for studies in mouse models, but validate in the specific tissue or cell type of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of validated raw materials, including polyclonal and monoclonal antibodies, antibody pairs, antigens, and ancillary reagents. For research involving the NF-κB pathway and IKK complex, we offer optimized anti-IKKγ antibodies, related signaling pathway products, and customized bulk sourcing services. From assay development to kit manufacturing, CamelBio is your reliable partner in accelerating diagnostic innovation.