Anti-IKKγ Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6K9

Core Product Specifications and Parameters

Biological Background: IKKγ Function and Localization

• IKKγ (NF-kappa-B essential modulator, NEMO), encoded by the IKBKG gene, functions as the regulatory subunit of the IKK core complex, phosphorylating NF-kappa-B inhibitors to trigger their degradation and activate NF-kappa-B. Related references: PMID:14695475 PMID:20724660 PMID:21518757
• It binds both Lys-63-linked and linear polyubiquitin chains, with a strong preference for linear polyubiquitin, critical for IKK activation by diverse signaling pathways. Related references: PMID:16547522 PMID:18287044 PMID:19033441
• Essential for viral activation of IRF3 and participates in TLR3/IFIH1-mediated antiviral innate immunity through Lys-27-linked polyubiquitination. Related references: PMID:19854139 PMID:20724660
• Implicated in NF-kappa-B-dependent protection from cytokine toxicity and acts as a mediator for HTLV-1 Tax oncoprotein-induced NF-kappa-B activation.
• Localizes to both cytoplasm and nucleus, and is broadly expressed across heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Keywords highlight alternative splicing, coiled coil and zinc-finger domains, phosphorylation, ubiquitination, and associations with ectodermal dysplasia and osteopetrosis.

Experimental Guidance and Technical Tips

• Due to alternative splicing, multiple WB bands may be seen around 48 kDa, 56 kDa, and 37 kDa; adjust exposure times accordingly.
• The immunogen (aa 250–390) covers the leucine zipper and part of the zinc finger domain, aiding detection of C-terminal variants.
• Validate in human, mouse, and rat samples as cross-reactivity suggests; HeLa or Jurkat cell lysates are suitable human positive controls.
• For IHC-P and IF/ICC, optimize antigen retrieval and antibody dilution based on tissue type and fixation conditions.
• Use appropriate blocking strategies to reduce non-specific background, especially when studying ubiquitin-dependent signaling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic and research use. For scientists investigating NF-kappa‑B signaling and IKK complex dynamics, CamelBio ensures a reliable supply of high‑quality reagents, including rare‑target raw materials, to support your development pipeline from concept to clinic.