Anti-IKKγ Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9Y6K9

Core Product Specifications and Parameters

Biological Background: IKKγ Function and Localization

• Regulatory subunit of the IKK core complex that phosphorylates NF-κB inhibitors, leading to inhibitor degradation and subsequent NF-κB activation. Related references: PMID:14695475, PMID:20724660, PMID:21518757
• Recognizes and binds both Lys-63-linked and linear polyubiquitin chains, with a higher affinity for linear chains, playing a key role in IKK activation upon cell stimulation. Related references: PMID:16547522, PMID:18287044, PMID:19033441
• Essential for viral activation of IRF3 and mediates TLR3- and IFIH1-dependent antiviral innate immune responses, a function requiring Lys-27-linked polyubiquitination. Related references: PMID:19854139, PMID:20724660
• Acts as a mediator for HTLV-1 Tax oncoprotein activation of NF-κB.
• Localizes to both the cytoplasm and nucleus.
• Widely expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Contains a coiled-coil domain and a zinc-finger motif; undergoes phosphorylation, ubiquitination, and disulfide bond formation; mutations are associated with ectodermal dysplasia and osteopetrosis.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa1-419) of human IKKγ, suggesting that the antibody may target an epitope within this domain. Consider mapping studies for detailed epitope characterization.
• Multiple bands around 48 kDa, 56 kDa, and 37 kDa may be observed in WB due to alternative splicing or post-translational modifications. Use appropriate protein ladders and validate with known controls.
• For IHC-P, optimize antigen retrieval and detection conditions, and validate staining with tissue samples known to express IKKγ.
• In IF/ICC, verify signal specificity by comparing with knockdown or knockout controls.
• Cross-reactivity with mouse and rat enables translational studies; however, sequence alignment may reveal subtle interspecies variations, so confirm reactivity in the relevant model system.
• Suitable for ELISA; consider pairing with a detection antibody for sandwich ELISA development after evaluating binding affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For research on NF-κB signaling and IKKγ-related pathways, we facilitate procurement of high-quality antibodies, recombinant proteins, and rare target reagents to support your assay development needs. Our integrated supply capabilities can help streamline your transition from concept to clinic.