Anti-IKKε Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14164

Core Product Specifications and Parameters

Biological Background: IKKε Function and Localization

• IKKε (Inhibitor of nuclear factor kappa-B kinase subunit epsilon), also known as IKK-i, is a serine/threonine kinase encoded by the IKBKE gene. Alternative splicing produces multiple isoforms.
• It plays a central role in regulating inflammatory responses to viral infection by activating type I IFN, NF-kappa-B, and STAT signaling pathways.
• Upon viral RNA sensor activation, IKKε associates with DDX3X and phosphorylates IRF3 and IRF7, promoting their homodimerization, nuclear translocation, and transcriptional activation of antiviral genes like IFNB.
• IKKε forms stimulus-dependent complexes with scaffold proteins including IPS1/MAVS, TANK, AZI2/NAP1, and TBKBP1/SINTBAD, modulating signaling specificity.
• It also regulates NF-kappa-B signaling by phosphorylating CYLD and NF-kappa-B inhibitors, leading to inhibitor degradation and NF-kappa-B activation.
• Subcellular localization includes the cytoplasm, nucleus, and PML nuclear bodies, consistent with its roles in signal transduction and transcriptional regulation.
• IKKε is highly expressed in spleen, thymus, peripheral blood leukocytes, pancreas, and placenta, with weaker expression in lung, kidney, prostate, ovary, and colon.
• In obesity, IKKε sustains chronic low-grade inflammation, negatively impacting insulin sensitivity, and also protects cells from DNA damage-induced death.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 at the N-terminus of human IKKε; ensure that your target sequence is present in the samples being tested.
• For western blot, the predicted molecular weight is ~80 kDa; consider using appropriate gel percentages for clear resolution.
• This antibody has been reported for detection in WB, IHC-P, and ELISA; validate in the relevant sample system and species (human, mouse, rat) as cross-reactivity may vary with sample preparation.
• In IHC-P, optimize antigen retrieval conditions and antibody dilution because nuclear and cytoplasmic staining patterns are expected based on IKKε localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic developers, labs, and research institutes with high-quality critical reagents. This anti-IKKε antibody supports research into antiviral innate immunity, inflammation, and metabolic disease, areas where validated immunoassay reagents are in demand. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets—helping you streamline procurement from concept to clinic.