Anti-IKKε (IKBKE) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14164

Core Product Specifications and Parameters

Biological Background: IKKε (IKBKE) Function and Localization

• IKKε (IKBKE) is a serine/threonine kinase that mediates inflammatory and antiviral responses by activating type I interferon, NF-κB, and STAT signaling pathways.
• Upon viral infection, IKKε interacts with RNA sensors (e.g., RIG-I) and adaptors (e.g., MAVS, TANK) to phosphorylate IRF3 and IRF7, inducing their dimerization and nuclear translocation to drive antiviral gene expression.
• IKKε also phosphorylates STAT1 at Ser-708, promoting the formation of ISGF3 complexes (STAT1-STAT2-IRF9) and the induction of interferon-stimulated genes (ISGs) with direct antiviral activity.
• Beyond immunity, IKKε contributes to energy homeostasis by sustaining obesity-related chronic inflammation, which negatively impacts insulin sensitivity, and phosphorylates AKT1.
• Subcellularly, IKKε localizes to the cytoplasm, nucleus, and PML nuclear bodies, reflecting its dynamic roles in signal transduction.
• The protein is highly expressed in lymphoid tissues (spleen, thymus, peripheral blood leukocytes) and the pancreas/placenta, with lower levels in lung, kidney, prostate, ovary, and colon.
• Post-translational modifications include phosphorylation and polyubiquitination, which are critical for its activation in response to TNFα and IL-1.
• Alternative splicing generates multiple isoforms, adding to the functional diversity of IKKε.

Experimental Guidance and Technical Tips

• The antibody is expected to detect a band at ~80 kDa by Western blot; verify band size using positive control lysates (e.g., Jurkat, THP-1, or spleen tissue).
• For IHC-P and IF/ICC, optimize fixation and antigen retrieval conditions; citrate buffer (pH 6.0) with heat-induced epitope retrieval is commonly effective.
• When studying IKKε activation, consider combining this antibody with phospho-specific or activation-dependent probes to assess phosphorylation status.
• Validate cross-reactivity in mouse and rat tissues using appropriate species-specific controls and note potential species-dependent background.
• For ELISA applications, this polyclonal antibody may be used as a capture or detection reagent; titrate to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, offering validated antibodies and auxiliary reagents to accelerate diagnostic development. This IKKε antibody supports research into inflammatory diseases, antiviral responses, and metabolic disorders. Beyond this catalog antibody, we provide customized rabbit polyclonal and monoclonal antibody development, matched antibody pairs, and bulk reagents to meet your scale-up and regulatory needs.