Anti-IKKε (IKBKE) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14164

Core Product Specifications and Parameters

Biological Background: IKKε Function and Localization

• IKKε (Inhibitor of nuclear factor kappa-B kinase subunit epsilon), encoded by IKBKE, is a serine/threonine kinase also known as inducible IκB kinase (IKK-i).
• Upon activation of viral RNA sensors (e.g., RIG-I-like receptors), IKKε associates with DDX3X and phosphorylates IRF3 and IRF7, triggering their dimerization and nuclear translocation to induce type I interferon and pro-inflammatory genes.
• IKKε forms distinct signaling complexes by recruiting scaffold proteins such as MAVS, TANK, NAP1/SINTBAD, enabling context-dependent transduction of antiviral and inflammatory signals.
• It regulates NF-κB signaling by phosphorylation of CYLD and IκB inhibitors, promoting dissociation of the NF-κB complex and subsequent nuclear activity.
• IKKε dynamically localizes to the cytoplasm, nucleus, and PML nuclear bodies, supporting its versatile role in signal transduction.
• The kinase also phosphorylates STAT1 at Ser-708, modulating the balance between type I (ISGF3) and type II (GAF) interferon responses.
• Beyond immunity, IKKε sustains chronic low-grade inflammation in obesity, negatively impacting insulin sensitivity, and protects against DNA damage-induced cell death.
• Highly expressed in spleen, thymus, and peripheral blood leukocytes; lower in lung, kidney, prostate, ovary, and colon.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 495–716) of human IKKε. Validate antibody specificity with positive controls (e.g., spleen lysate) and negative controls in your model system.
• In Western blot, expect a band at ~80 kDa. Use appropriate molecular weight markers and verify the band size.
• For IHC-P, consider antigen retrieval (e.g., citrate pH 6.0) and optimize dilution using tissues with known IKKε expression (spleen, thymus).
• This antibody cross-reacts with mouse and rat IKKε; confirm sequence homology before application.
• Polyclonal antibodies may exhibit lot-to-lot variability; include reference controls in each experiment.
• Validated applications: WB, IHC-P, ELISA. Not tested in other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic developers from concept to clinic. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents for targets like IKKε in innate immunity and inflammation research. Contact us for bulk orders or customized sourcing of rare-target reagents.