Anti-IKKα Rabbit Monoclonal Antibody for WB, ELISA - O15111

Core Product Specifications and Parameters

Biological Background: IKKα Function and Localization

• Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKKα), also known as CHUK, IKK1, IKKA, or TCF-16, is a serine/threonine kinase essential for NF-kappa-B signaling activated by inflammatory cytokines, bacterial/viral products, or cellular stress. Related references: PMID:18626576 PMID:9244310 PMID:9252186

• In the canonical NF-κB pathway, IKKα phosphorylates inhibitors of NF-κB (IκBs) on serine residues, triggering ubiquitin-dependent degradation and freeing NF-κB to translocate to the nucleus and activate transcription of immune and survival genes. Related references: PMID:18626576 PMID:9244310 PMID:9252186

• As part of the non-canonical NF-κB pathway, CHUK/IKKα homodimer phosphorylates NFKB2/p100 to promote its processing to p52 and the formation of transcriptionally active RelB-p52 complexes, regulating B-cell survival and lymphoid organogenesis. Related references: PMID:20501937

• IKKα negatively regulates NF-κB signaling by phosphorylating TAXBP1 to assemble the A20 ubiquitin-editing complex and by destabilizing MAP3K14/NIK in the non-canonical pathway, providing critical feedback control. Related references: PMID:21765415

• In the nucleus, IKKα directly phosphorylates histone H3 at Ser-10 and acetyltransferase CREBBP, enhancing chromatin accessibility and transcriptional activity at NF-κB-responsive promoters. Related references: PMID:12789342 PMID:17434128

• Additional substrates include FOXO3 (pro-apoptotic transcription factor), RIPK1 (necroptosis regulation), and AMBRA1 (mitophagy), highlighting diverse cellular roles. Related references: PMID:15084260 PMID:30217973

• IKKα is widely expressed and localizes to both cytoplasm and nucleus, where it undergoes regulatory acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from CHUK; thus, this monoclonal antibody may recognize endogenous IKKα in human, mouse, and rat samples. Consider validating in your specific cell lines and tissues.
• For Western blotting, a band at approximately 85 kDa is expected under reducing conditions. Optimize antibody dilution and sample loading based on IKKα expression levels in your system.
• For ELISA, pair with a suitable capture/detection system after determining the optimal coating concentration and incubation conditions.
• Since IKKα is involved in diverse signaling complexes, consider performing co-immunoprecipitation or pull-down assays carefully, as epitope accessibility may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined, one-stop source for validated IVD raw materials. Whether you need optimized monoclonal antibodies like anti-IKKα, complementary antibody pairs, bulk ancillary reagents, or rare target sourcing for NF-κB pathway studies, our integrated supply chain and technical support accelerate your development from concept to clinic.