Anti-IKK beta Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - O14920

Core Product Specifications and Parameters

Biological Background: IKK beta Function and Localization

• Protein identity: IKK beta (IKBKB) is a serine/threonine protein kinase also known as IKK-2, NFKBIKB, or IKK-beta, and is a core catalytic subunit of the IκB kinase (IKK) complex.
• NF-κB signaling: It plays an essential role in the canonical NF-κB pathway, activated by pro-inflammatory cytokines, pathogens, or cellular stress, leading to phosphorylation and subsequent ubiquitin-dependent degradation of IκB inhibitors and nuclear translocation of NF-κB (PubMed:20434986, PubMed:20797629, PubMed:21138416). Related references: PMID:20434986, PMID:20797629, PMID:21138416
• Substrate diversity: Beyond IκB, IKK beta phosphorylates NEMO/IKBKG, NF-κB subunits RELA and NFKB1, and IKK-related kinases TBK1/IKBKE, providing a negative feedback mechanism that prevents excessive inflammation (PubMed:11297557, PubMed:20410276). Related references: PMID:11297557, PMID:20410276
• Additional regulatory roles: IKK beta phosphorylates FOXO3 to inhibit apoptosis, modifies RIPK1 to suppress TNF-induced cell death, activates IRF5 for interferon induction, and phosphorylates STAT1 to promote inflammatory gene expression (PubMed:15084260, PubMed:25326418, PubMed:32209697, PubMed:38621137). Related references: PMID:15084260, PMID:25326418, PMID:32209697, PMID:38621137
• Subcellular localization: Found in the cytoplasm, nucleus, and membrane rafts, consistent with its dynamic role in signal transduction.
• Tissue expression: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, and peripheral blood.
• Disease relevance: Mutations in IKBKB are associated with severe combined immunodeficiency (SCID) and other immune disorders, underscoring its critical role in immune function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 261-411, targeting a central region of human IKK beta. This design may recognize multiple isoforms or cleavage products, as suggested by the broad observed molecular weight range (29–87 kDa).
• For Western blot, validate the antibody in relevant human cell lines (e.g., HeLa, Jurkat) and use appropriate negative controls. Consider protease inhibitors during lysis to minimize degradation.
• In immunoprecipitation (IP), the antibody can pull down the IKK complex; confirm co-precipitation of NEMO/IKBKG if studying complex assembly.
• For immunofluorescence (IF/ICC), expect cytosolic and nuclear staining; membrane raft localization may require specialized fixation/permeabilization protocols.
• ELISA applications should use a validated standard curve with recombinant IKK beta protein for quantitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, to support your diagnostic development from concept to clinic. This IKK beta monoclonal antibody, critical for NF-κB signaling and inflammation research, is part of our commitment to providing high-quality, ready-to-use reagents. Through our extensive network and technical expertise, we can also source rare-target raw materials and assist with custom antibody generation, ensuring you have reliable solutions for every stage of your project.