Anti-IKBIP Polyclonal Antibody for WB, IF/ICC, ELISA - Q70UQ0

Core Product Specifications and Parameters

Biological Background: IKBIP Function and Localization

• IKBIP (inhibitor of nuclear factor kappa-B kinase-interacting protein), also known as IKIP, is a target of p53/TP53 and functions as a pro-apoptotic protein.
• The protein is anchored in the endoplasmic reticulum membrane, with predicted transmembrane helices, consistent with its role in stress-induced apoptosis.
• Alternative splicing produces multiple isoforms; isoform 4 is expressed in tissues including lung, kidney, spleen, thymus, and skeletal muscle.
• IKBIP is annotated as a glycoprotein, suggesting post-translational modification that may affect its stability or localization.
• The canonical sequence (UniProt Q70UQ0) has a calculated molecular mass of ~39 kDa, matching the observed protein weight.
• It is expressed in vein endothelial cells, indicating potential involvement in vascular pathophysiology.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 115-350) spans a region likely containing functional domains. For Western blotting, use reducing conditions and probe with appropriate controls; the expected band is around 39 kDa.
• For immunofluorescence/immunocytochemistry, optimize permeabilization to access the ER membrane; co-staining with an ER marker is advisable.
• In ELISA, perform checkerboard titrations to determine optimal coating and detection concentrations. Cross-reactivity with mouse and rat homologs should be confirmed in the specific assay format.
• Since IKBIP is p53-inducible, consider treating cells with genotoxic agents to upregulate expression prior to detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-IKBIP antibody, to accelerate your apoptosis and endothelial biology research. From antibody pairs to bulk reagents and rare target sourcing, we support diagnostic developers with reliable supply chains and technical expertise.