Anti-IκBα Rabbit Monoclonal Antibody for WB, ELISA - Q63746

Core Product Specifications and Parameters

Biological Background: IκBα Function and Localization

• IκBα (NF-kappa-B inhibitor alpha) is a key regulator of the NF-κB signaling pathway, primarily functioning to retain NF-κB dimers in the cytoplasm.
• It contains multiple ankyrin repeat domains that mediate protein-protein interactions, particularly with the RELA/p65 and NFKB1/p50 subunits of NF-κB.
• Through its ankyrin repeats, IκBα masks the nuclear localization signals (NLS) of NF-κB, preventing its translocation to the nucleus.
• Upon stimulation by pro-inflammatory cytokines or immune signals, IκBα is phosphorylated, triggering its ubiquitination and subsequent proteasomal degradation.
• Degradation of IκBα releases NF-κB, allowing it to enter the nucleus and activate transcription of target genes involved in immune and inflammatory responses.
• IκBα shuttles between the cytoplasm and nucleus; it can also enter the nucleus to remove NF-κB from DNA and export it back to the cytoplasm.
• The protein undergoes various post-translational modifications including phosphorylation, ubiquitination, and hydroxylation, which regulate its stability and function.
• Keywords: ANK repeat, cytoplasm, hydroxylation, isopeptide bond, nucleus, phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (aa 1-314), which may provide high specificity and affinity for detecting endogenous IκBα.
• For Western blot, a band around 35 kDa is expected; validate in relevant cell or tissue lysates from mouse or rat.
• Consider using this antibody in ELISA applications; it is supplied as a monoclonal antibody from rabbit, ensuring batch-to-batch consistency.
• Since IκBα is rapidly degraded upon pathway activation, include appropriate inhibitors and controls in your experimental design.
• Cross-reactivity has been confirmed with mouse and rat samples; for other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined sourcing solution for high-quality IVD raw materials. This IκBα antibody supports NF-κB signaling research, and we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Contact us for rare target raw-material sourcing to accelerate your assay development.