Anti-IκBα Polyclonal Antibody for WB, ELISA - P25963

Core Product Specifications and Parameters

Biological Background: IκBα Function and Localization

• IκBα inhibits NF-κB/REL complexes by trapping RELA/p65 and NFKB1/p50 dimers in the cytoplasm, masking their nuclear localization signals.
  Related references: PMID:1493333 PMID:36651806 PMID:7479976
• Upon immune or pro-inflammatory stimulation, it becomes phosphorylated, ubiquitinated, and degraded, enabling RELA nuclear translocation and transcription activation.
  Related references: PMID:7479976 PMID:7628694 PMID:7796813
• Subcellularly, IκBα is predominantly cytoplasmic but undergoes nucleocytoplasmic shuttling to control NF-κB activity.
• The protein contains multiple ankyrin repeats and is subject to post-translational modifications including phosphorylation, ubiquitination, and hydroxylation.
• Mutations in NFKBIA are associated with ectodermal dysplasia and immune deficiency (EDAID2), highlighting its role in immune and epithelial homeostasis.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against a synthetic peptide from the C-terminal region (aa 166-265) of human IκBα; it may detect both unmodified and modified forms, but validation in your system is advised.
• Suitable for Western blot (WB) and ELISA; a band at ~36 kDa is expected in WB, though mobility may shift due to phosphorylation.
• Cross-reactivity is reported with human, mouse, and rat IκBα; include appropriate species controls.
• Optimize antibody dilution for each assay and confirm specificity with siRNA knockdown, knockout lysates, or blocking peptide controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-IκBα polyclonal antibody supports research into NF-κB signaling, inflammation, and immune regulation. Our global sourcing network provides reliable access to rare-target materials, accelerating your diagnostic development from concept to clinic.