Anti-IκBα Monoclonal Antibody for WB, IHC-P, ELISA - P25963

Core Product Specifications and Parameters

Biological Background: IκBα Function and Localization

• NF-kappa-B inhibitor alpha (IκBα), also known as I-kappa-B-alpha or MAD3, is encoded by the NFKBIA gene in humans.
• IκBα inhibits NF-kappa-B/REL complexes by retaining RELA/p65 and NFKB1/p50 dimers in the cytoplasm through masking their nuclear localization signals. Related references: PMID:1493333, PMID:36651806, PMID:7479976
• Upon immune and pro-inflammatory stimulation, IκBα is phosphorylated, ubiquitinated, and degraded, allowing the RELA dimer to translocate to the nucleus and activate transcription. Related references: PMID:7479976, PMID:7628694, PMID:7796813
• IκBα localizes to both the cytoplasm and nucleus, shuttling between compartments to regulate NF-kB activity.
• Mutations in NFKBIA are associated with ectodermal dysplasia and other immunodeficiencies.
• The protein structure contains ANK repeats and is a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen sequence lies within the N-terminal 1-100 amino acids of human IκBα; consider sequence alignment across species if cross-reactivity beyond human, mouse, and rat is desired.
• For Western blot (WB), the predicted molecular weight is 36 kDa; validate with appropriate positive and negative controls.
• For immunohistochemistry paraffin (IHC-P), perform antigen retrieval and optimize antibody titration for each tissue type.
• The antibody is suitable for ELISA; consider pairing with a capture antibody for sandwich ELISA format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers seamless access to critical raw materials for diagnostic development, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. This anti-IκBα rabbit monoclonal antibody is a valuable tool for NF-kB signaling and immuno-inflammation research, supporting assay development from concept to clinic. Our supply solutions cover bulk orders, rare targets, and custom sourcing to meet your IVD manufacturing needs.