Anti-Ikaros Rabbit Polyclonal Antibody for WB, ELISA - Q13422

Core Product Specifications and Parameters

Biological Background: Ikaros Function and Localization

• DNA-binding protein Ikaros (IKZF1) acts as a transcription regulator essential for hematopoietic cell differentiation, primarily during lymphocyte development and erythroid maturation.
  Related references:
  PMID:17934067
• Binds gamma-satellite DNA, contributing to chromatin organization and gene silencing.
  Related references:
  PMID:17135265 PMID:19141594
• Functions are isoform-specific, with dominant-negative isoforms modulating transcriptional activity; alternative splicing generates variants with distinct DNA-binding and regulatory properties.
• Localizes predominantly in the nucleus but can also be detected in the cytoplasm, consistent with its roles in transcription and chromatin remodeling.
• Recruits chromatin-remodeling complexes (NuRD and BAF) to target loci, such as the beta-globin gene, integrating epigenetic regulation with lineage-specific gene expression.
• Highly expressed in lymphoid tissues (thymus, spleen, peripheral blood leukocytes, lymph nodes) and at lower levels in bone marrow and small intestine, reflecting its hematopoiesis-specific roles.
• Post-translationally modified by phosphorylation and ubiquitin-like conjugation (isopeptide bonds), which may influence protein stability and transcriptional activity.
• Keywords from UniProt highlight its involvement in cell cycle, chromatin regulation, and developmental processes, with documented disease associations and chromosomal rearrangements.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 296–457 of human Ikaros (NP_006051.1), covering the C‑terminal region that is conserved across multiple isoforms; consider using isoform-specific controls in your assays.
• For Western blotting (WB), validate antibody specificity in human lymphocyte or erythroid cell lines (e.g., Jurkat, K562) and use appropriate loading controls.
• In ELISA, titrate the antibody carefully and include negative controls (e.g., Ikaros‑knockout lysates) to confirm signal specificity.
• Because Ikaros is subject to alternative splicing and post‑translational modifications, observed band sizes may differ from the theoretical 58 kDa; run reducing and non‑reducing gels to distinguish monomeric and modified forms.
• Cross‑reactivity is reported only for human Ikaros; perform sequence alignment before testing in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents, streamlining your diagnostic development process. This anti‑Ikaros polyclonal antibody supports research in hematopoiesis, lymphocyte biology, and related disorders. For specialized or rare‑target antibody sourcing, contact our team to discuss customized solutions.