Anti-Ikaros Monoclonal Antibody for WB, IF/ICC, ELISA, ChIP, CUT&Tag - Q13422

Core Product Specifications and Parameters

Biological Background: Ikaros Function and Localization

• Ikaros (IKZF1) is a DNA-binding zinc-finger transcription factor that belongs to the Ikaros family, also known as lymphoid transcription factor LyF-1. Multiple isoforms are produced by alternative splicing, some acting as dominant-negative regulators (PubMed:17135265, PubMed:17934067). Related references: PMID:17135265 PMID:17934067
• It functions as a key regulator of hematopoietic cell differentiation, playing a critical role in lymphocyte development, particularly B- and T-cells. It binds and activates the enhancer of the CD3-delta gene and represses the TDT gene during thymocyte differentiation (PubMed:17934067). Related references: PMID:17934067
• Ikaros modulates chromatin structure by recruiting chromatin-remodeling complexes such as NuRD and BAF (SWI/SNF) to target loci, including the beta-globin locus in adult erythrocytes, forming the PYR complex.
• It binds to gamma-satellite DNA, contributing to pericentromeric heterochromatin organization (PubMed:17135265, PubMed:19141594). Related references: PMID:17135265 PMID:19141594
• Subcellular localization is primarily nuclear, consistent with its role in transcriptional regulation, but cytoplasmic detection has also been reported.
• Abundantly expressed in lymphoid tissues such as thymus, spleen, peripheral blood leukocytes, and lymph nodes, with lower levels in bone marrow and small intestine.
• The protein undergoes post-translational phosphorylation and ubiquitin-like conjugation, which may regulate its activity and stability. It contains multiple C2H2-type zinc fingers for DNA binding and protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 250-350 of human Ikaros, a region outside the zinc-finger domains. This may allow detection of multiple isoforms.
• For western blotting (WB), the predicted molecular weight is approximately 58 kDa, but isoform-specific bands may appear at different sizes. Validate band patterns in relevant cell lysates (e.g., Jurkat, thymus).
• For chromatin immunoprecipitation (ChIP) and CUT&Tag assays, due to Ikaros's association with chromatin, it is advisable to optimize fixation conditions and validate enrichment at known target genes (e.g., CD3-delta, gamma-satellite) in hematopoietic cells.
• In immunofluorescence (IF) and immunocytochemistry (ICC), expect predominantly nuclear staining. Co-staining with a nuclear marker is recommended.
• The antibody shows cross-reactivity with mouse samples, which may be advantageous for translational studies; however, sequence alignment of the immunogen region is suggested before use in mouse models.

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CamelBio partners with diagnostic manufacturers and research laboratories to streamline the sourcing of validated IVD raw materials. Whether you require optimized monoclonal antibodies, like the Ikaros Rabbit mAb for hematopoiesis research, or need consistent bulk supply of ancillary reagents, we provide comprehensive procurement support. Our portfolio covers rare-target antibodies, recombinant proteins, and custom development services to accelerate your assay development from concept to clinic.