Anti-IK Polyclonal Antibody for WB, ELISA - Q13123

Core Product Specifications and Parameters

Biological Background: IK (Red/RER) Function and Localization

• IK (also known as Protein Red, RER, or Cytokine IK) is a ubiquitously expressed nuclear protein encoded by the IK gene in humans.
• Functions as a core component of the spliceosome, involved in pre-mRNA splicing (PubMed:28781166). Related references: PMID:28781166
• Acts as an auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species.
• Required for normal mitotic cell cycle progression by recruiting MAD1L1 and MAD2L1 to kinetochores, thereby triggering the spindle assembly checkpoint (PubMed:22351768, PubMed:24252166). Related references: PMID:22351768, PMID:24252166
• Required for normal accumulation of SMU1 protein (PubMed:24945353). Related references: PMID:24945353
• Localizes to the nucleus, nucleoplasm, chromosomes, cytoplasm, and spindle poles, reflecting its dynamic roles in splicing and mitosis.
• Undergoes phosphorylation, acetylation, and ubiquitination (isopeptide bond), which may regulate its activity and interactions.
• Together with SMU1, mediates normal splicing of influenza A virus NS1 pre-mRNA, essential for viral particle production.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1–280 amino acids of human IK; consider potential epitope accessibility in fixed or native conformations.
• For Western blot, a band corresponding to the predicted molecular weight of ~66 kDa is expected; validate the optimal antibody dilution using a relevant cell or tissue lysate.
• Due to the polyclonal nature, minor additional bands may appear; include appropriate controls and confirm specificity in the relevant sample system.
• The reported cross-reactivity with mouse and rat makes this antibody suitable for comparative studies across these species; however, verify reactivity in your specific experimental setup.
• For ELISA applications, consider using the antibody as a capture or detection reagent after establishing optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality antibodies like this anti-IK polyclonal antibody, supporting spliceosome and cell cycle research. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities, all designed to accelerate your assay development from concept to clinic.