Anti-IHH Rabbit Polyclonal Antibody for WB and ELISA - Q14623

Core Product Specifications and Parameters

Biological Background: IHH Function and Localization

• IHH (Indian hedgehog protein) is a member of the hedgehog family, encoded by the IHH gene. The full-length precursor undergoes autoproteolytic cleavage and cholesterol modification to generate the active N-product morphogen.
• The N-product of IHH activates the smoothened signaling pathway by binding to patched receptors (PTCH1/2), relieving SMO inhibition, which is essential for bone and cartilage development.
• IHH positively regulates PTHLH expression to prevent chondrocyte hypertrophy during endochondral bone formation, and independently promotes chondrocyte proliferation.
• IHH plays a role in hedgehog paracrine signaling (PMID:24342078) and, when associated with VLDL particles, acts as a circulating morphogen to maintain endothelial cell integrity (PMID:20839884). Related references: PMID:24342078 PMID:20839884
• Beyond skeletal development, IHH is involved in the development of retinal pigment epithelium, photoreceptors, and periocular tissues.
• Subcellularly, the protein resides in the endoplasmic reticulum and Golgi apparatus membranes; the secreted N-product can associate with the cell membrane.
• Tissue expression is observed in embryonic lung, and in adult kidney and liver.
• Key post-translational modifications include autocatalytic cleavage, cholesterol attachment, and palmitoylation; the protein is classified as a glycoprotein and lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 202–411 of human IHH, covering a region distal to the autocatalytic cleavage site. The full-length protein has a predicted molecular weight of 45 kDa, but consider that endogenous IHH may be processed, resulting in additional bands in WB.
• For Western blot, validate antibody specificity using appropriate positive controls (e.g., tissues or cells known to express IHH) and consider using a loading control. Optimize blocking and detection conditions empirically.
• This polyclonal antibody reacts with human, mouse, and rat IHH, making it suitable for cross-species studies. However, confirm reactivity in the target species if not previously validated.
• For ELISA, titration of the antibody and optimal coating antigen concentrations should be determined experimentally.
• As with any polyclonal reagent, lot-to-lot variability may occur; consider reserving a calibration standard for consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined channel for sourcing high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies like this IHH antibody. Whether you need optimized antibody pairs, bulk ancillary reagents, or assistance with rare target sourcing for bone development or cell signaling research, our one-stop platform bridges the gap from concept to clinic. Contact us to discuss your specific raw material requirements.