Anti-IGSF3 Rabbit Polyclonal Antibody for WB, ELISA - O75054

Core Product Specifications and Parameters

Biological Background: IGSF3 Function and Localization

• IGSF3 (Immunoglobulin superfamily member 3), also designated EWI-3 (Glu-Trp-Ile EWI motif-containing protein 3), is a transmembrane glycoprotein encoded by the IGSF3 gene (Gene ID: 3321). Synonyms include V8 and LCDD.
• The protein belongs to the immunoglobulin superfamily and contains multiple immunoglobulin-like domains in its extracellular region, along with a single-pass transmembrane helix and a cytoplasmic tail.
• IGSF3 is broadly expressed across human tissues, with notably high levels in placenta, kidney, and lung, as indicated by proteomics and transcriptomic data.
• As a membrane protein, IGSF3 is predicted to localize at the plasma membrane, consistent with a potential role in cell adhesion or immune recognition, although its precise function remains to be fully elucidated.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are typical for Ig-superfamily members and may contribute to protein stability and ligand interaction.
• Alternative splicing generates multiple transcript variants, potentially yielding isoforms with distinct structural and functional properties.
• The gene has been associated with chromosomal rearrangements, suggesting possible involvement in pathological contexts such as cancer.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein spanning amino acids 350–562 of human IGSF3 (NP_001007238.1), corresponding to a portion of the extracellular region. It is expected to recognize multiple epitopes and possibly multiple isoforms.
• For Western blotting (WB), a band corresponding to the full-length protein is expected around 135 kDa. Additional bands from splice variants or post-translational modifications may be observed. Validate using appropriate positive (e.g., placental or kidney lysate) and negative controls.
• For ELISA applications, use purified recombinant IGSF3 protein as a standard to establish linear range and detection limit. Cross-reactivity with other species has not been tested; reactivity is confirmed for human samples.
• Given the predicted membrane localization, consider using membrane-enriched protein fractions for improved detection sensitivity in WB.
• As with all polyclonal antibodies, lot-to-lot variability may exist; it is recommended to validate each new lot in your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For IGSF3-related research and assay development, we supply high-quality reagents like this anti-IGSF3 polyclonal antibody, alongside technical consultation to support your journey from concept to clinic. Contact us to explore custom sourcing solutions tailored to your workflow.