Anti-IGHMBP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P38935

Core Product Specifications and Parameters

Biological Background: IGHMBP2 Function and Localization

• IGHMBP2 is a probable ATP-dependent 5′-to-3′ helicase that unwinds both RNA and DNA duplexes; it has low processivity in vitro. Related references: PMID:19158098 PMID:30218034
• It specifically binds and unwinds 5′-phosphorylated single-stranded guanine-rich sequences. Related references: PMID:22999958 PMID:8349627
• It may play a role in RNA metabolism, ribosome biogenesis, or initiation of translation. Related references: PMID:19158098 PMID:19299493
• IGHMBP2 interacts with tRNA-Tyr, suggesting a function in tRNA processing or translational regulation. Related references: PMID:19299493
• The protein may also regulate transcription (by similarity).
• Subcellularly, it localizes to the nucleus, cytoplasm, and neuronal axons.
• IGHMBP2 is ubiquitously expressed with highest levels in the cerebellum, and is found in developing and adult brain.
• Mutations in IGHMBP2 are associated with neurodegenerative disorders including Charcot-Marie-Tooth disease type 2S (CMT2S) and spinal muscular atrophy with respiratory distress type 1 (SMARD1).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 540-720 of human IGHMBP2 (NP_002171.2), a conserved region; cross-reactivity with mouse and rat is supported.
• For Western blot, a band near the predicted molecular weight of 109 kDa is expected; optimize protein loading and transfer conditions.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization, and staining should be validated in tissues with known IGHMBP2 expression (e.g., cerebellum).
• Validate the antibody in the relevant sample system with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, streamlining development from concept to clinic. This anti-IGHMBP2 polyclonal antibody supports studies on RNA helicases, ribosome biogenesis, and neurodegenerative disease mechanisms. Complementing our antibody portfolio, we offer validated antibody pairs, recombinant proteins, and custom raw-material sourcing for rare or novel targets to meet your assay development needs.