Anti-IGHD Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P01880

Core Product Specifications and Parameters

Biological Background: IGHD Function and Localization

• IGHD encodes the constant region of immunoglobulin heavy chains, forming a core component of antibodies that mediate antigen elimination. Related references: PMID:20176268, PMID:22158414
• The antigen-binding site is generated through V-(D)-J rearrangement and somatic hypermutation, enabling affinity maturation upon antigen exposure. Related references: PMID:17576170, PMID:20176268
• IgD is coexpressed with IgM on the surface of most peripheral B cells, serving as a major antigen receptor; membrane-bound IgD triggers B-cell activation via CD79A/CD79B phosphorylation by Src kinases.
• Soluble IgD circulates at lower levels than IgG, IgA, and IgM but significantly higher than IgE, and its secretion is downregulated following antigen binding.
• IgD stimulates monocytes and peripheral blood mononuclear cells to produce pro-inflammatory cytokines such as TNF, IL1B, IL6, and IL10. Related references: PMID:10702483, PMID:11282392, PMID:8774350
• The protein exists as secreted and membrane-bound isoforms and localizes to the extracellular space and plasma membrane.
• IGHD is a glycoprotein with intrachain disulfide bonds and an immunoglobulin domain structure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the N-terminal region (amino acids 1–100) of human IGHD; consider this when designing experiments to ensure epitope availability.
• This antibody is suitable for Western blot (detecting a band at ~42 kDa), immunohistochemistry-paraffin, and ELISA; optimize dilutions for each application.
• For IHC-P, validate in appropriate human lymphoid tissues where IgD is expressed on B cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. Our IGHD-directed reagents are ideally suited for B-cell immunophenotyping and humoral immunity research applications. Contact us for rare target sourcing and customized technical support.