Anti-IGFBPL1 Rabbit Polyclonal Antibody for WB, ELISA - Q8WX77

Core Product Specifications and Parameters

Biological Background: IGFBPL1 Function and Localization

• Insulin-like growth factor-binding protein-like 1 (IGFBPL1), also known as IGFBP-related protein 10 or IGFBP-rP4, belongs to the IGFBP family and contains an immunoglobulin-like domain.
• IGFBPL1 binds insulin-like growth factors (IGFs), prolonging their half-life in circulation and modulating their interaction with cell surface receptors; it can either inhibit or stimulate IGF-mediated growth depending on the cellular context.
• The protein is secreted and likely functions extracellularly to regulate IGF bioavailability and downstream signaling pathways.
• IGFBPL1 is predominantly expressed in the brain and testis, with lower levels detected in the prostate, bladder, and lung, suggesting tissue-specific roles in growth regulation and possibly neuroendocrine functions.
• Post-translational modifications include disulfide bond formation and glycosylation, which may influence protein stability and ligand binding; the protein possesses a signal peptide for secretion.
• IGFBPL1 has been proposed as a putative tumor suppressor, potentially through its IGF-regulatory activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning residues 150–250 of human IGFBPL1; this region lies outside the predicted signal peptide and may afford good accessibility for antibody recognition.
• For Western blotting, under reducing conditions, the primary band is expected around 29 kDa, but higher molecular weight bands may appear due to glycosylation or dimerization; validate findings with appropriate controls.
• In ELISA applications, consider using the antibody in a sandwich format paired with another IGFBPL1-specific antibody to capture and detect the analyte; cross-reactivity with mouse and rat isoforms supports comparative studies.
• Include relevant positive control lysates (e.g., brain or testis tissue from human, mouse, or rat) to confirm specificity; negative controls with IGFBPL1 knockout samples are recommended where possible.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted IVD raw material partner, CamelBio connects diagnostic manufacturers and research teams with high-quality antibodies, reagents, and technical support from concept to clinic. For IGF signaling and tumor suppression research, we offer validated polyclonal and monoclonal antibodies, bulk production, and ancillary reagents to accelerate your assay development. Contact us to explore tailored sourcing solutions for rare targets and established biomarkers alike.