Anti-IGFBP7 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q16270

Core Product Specifications and Parameters

Biological Background: IGFBP7 Function and Localization

• IGFBP7, also known as MAC25, PSF, TAF, or IBP-7, is a secreted insulin-like growth factor-binding protein with roles in cell adhesion, growth factor binding, and angiogenesis.
• It binds IGF1 and IGF2 with relatively low affinity and stimulates prostacyclin (PGI2) production, which may influence vasodilation and platelet aggregation.
• IGFBP7 functions as a ligand for CD93, contributing to angiogenic processes, as recently demonstrated. Related references: PMID:38218180
• The protein contains an immunoglobulin domain and is classified under the IGFBP family, but it has a lower molecular weight (~29 kDa) compared to classical IGFBPs.
• Subcellularly, IGFBP7 is secreted and found in the extracellular space, consistent with its role in cell adhesion and growth factor interaction.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which may affect its stability and function.
• Alternative splicing and RNA editing have been reported for this gene, potentially generating isoforms with distinct properties.
• Keywords from UniProt also highlight its involvement in cell adhesion, direct protein sequencing evidence, and its identification in proteomics studies.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from the N-terminal region (aa 50-150), which may confer specificity for the mature secreted form of IGFBP7.
• For Western blot (WB) detection, the predicted molecular weight is ~29 kDa; confirm band size against a positive control lysate.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider permeabilization protocols since IGFBP7 is secreted; staining may appear extracellular in non-permeabilized samples.
• For ELISA, validate coating and detection conditions; pairing with a different epitope-specific antibody may improve sensitivity.
• Cross-reactivity with mouse and rat orthologs is supported by supplier data; verify conservation of the epitope sequence across species.

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