Anti-IGFBP4 Rabbit Polyclonal Antibody - P22692

Core Product Specifications and Parameters

Biological Background: IGFBP4 Function and Localization

• IGFBP4 (Insulin-like growth factor-binding protein 4) is a secreted protein that modulates the bioavailability and activity of insulin-like growth factors (IGFs) by binding to them and prolonging their half-life.
• It can either inhibit or stimulate IGF-mediated cell growth, depending on the cellular context, by altering the interaction of IGFs with their cell surface receptors.
• The protein is characterized by a conserved IGF-binding domain and contains disulfide bonds that stabilize its structure, as noted in UniProt keywords.
• Post-translational modifications include glycosylation and phosphorylation, which may influence its stability and function.
• Alternative splicing generates multiple isoforms, contributing to functional diversity (UniProt).
• Subcellular localization is predominantly extracellular; it is secreted into the extracellular space where it exerts its regulatory effects.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 22-258 of human IGFBP4) targets the mature protein, so the antibody likely recognizes both native and denatured forms, supporting use in applications such as WB and IHC-P.
• Given cross-reactivity with mouse and rat, consider validating the antibody in these species' relevant tissue or cell samples for comparative studies.
• For ELISA setups, pairing this antibody with a different clone or a matched detection antibody may improve sensitivity; optimize coating and detection conditions.
• In IF/ICC, ensure proper fixation and permeabilization to allow antibody access to the secreted IGFBP4, as it may be localized in the extracellular matrix or culture supernatant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibodies like this anti-IGFBP4 polyclonal. Whether you require optimized antibody pairs, bulk ancillary reagents, or assistance sourcing rare-target raw materials, our platform streamlines your project from discovery to clinical application.