Anti-IGFBP4 Rabbit Polyclonal Antibody for WB, ELISA - P22692

Core Product Specifications and Parameters

Biological Background: IGFBP4 Function and Localization

• IGFBP4 is a secreted insulin-like growth factor-binding protein that prolongs the half-life of IGFs and modulates their interaction with cell surface receptors.
• It is encoded by the IGFBP4 gene, and alternative splicing generates multiple isoforms.
• The protein features conserved disulfide bonds, is glycosylated, and undergoes phosphorylation.
• Subcellular localization is predominantly secretory, consistent with its role in extracellular IGF transport and activity modulation.
• The calculated molecular weight is approximately 28 kDa (27934 Da).
• IGFBP4 belongs to the IGFBP family, which regulates IGF bioavailability and signaling in various tissues.

Experimental Guidance and Technical Tips

• For Western blotting, consider testing a dilution range of 1:500–1:2000 and validate with cell lysates from IGFBP4-expressing lines.
• For ELISA, pair the antibody with a suitable detection antibody and optimize coating antigen concentration.
• Cross-reactivity with mouse and rat IGFBP4 is reported; verify specificity in the appropriate sample system.
• Because IGFBP4 is secreted, detection may be enhanced by using serum or conditioned medium samples.
• The immunogen spans the C-terminal region (amino acids 178–258); confirm epitope accessibility in your application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, including validated antibodies, optimized polyclonal/monoclonal reagents, and bulk ancillary supplies. This anti-IGFBP4 product supports research in growth factor signaling and metabolic regulation. Our team bridges the gap from early development to clinical application with reliable sourcing and technical expertise.