Anti-IGFBP3 Rabbit Polyclonal Antibody for WB and ELISA - P47878

Core Product Specifications and Parameters

Biological Background: IGFBP3 Function and Localization

• Insulin-like growth factor-binding protein 3 (IGFBP3) prolongs the half-life of IGFs and modulates their interaction with cell surface receptors, thereby regulating cell growth.
• Depending on the cellular context, IGFBP3 can either inhibit or stimulate the growth-promoting effects of IGFs in culture.
• IGFBP3 exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R, including the promotion of testicular germ cell apoptosis.
• The protein is secreted and contains a signal peptide; it undergoes post-translational modifications including phosphorylation and glycosylation.
• Key structural features include multiple disulfide bonds and a conserved IGF-binding domain.
• UniProt keywords: Apoptosis, Disulfide bond, Glycoprotein, Growth factor binding, Phosphoprotein, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen covers the mature mouse IGFBP3 sequence (aa 28–292), suggesting the antibody recognizes native and recombinant protein forms.
• For Western blot, the predicted molecular weight is 32 kDa; however, glycosylation may result in bands migrating at higher molecular weights. Include a suitable loading control and consider de-glycosylation for precise size confirmation.
• In ELISA, validate the antibody’s performance as a capture or detection reagent using mouse or rat samples, and titrate to determine optimal working concentrations.
• Because the antibody is polyclonal and cross-reacts with rat, it is suitable for comparative studies between mouse and rat models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to optimized polyclonal antibodies and bulk ancillary reagents. For IGFBP3-focused projects, we offer tailored sourcing of anti-IGFBP3 antibodies and related reagents to streamline assay development, from concept to clinical application. Contact us to explore how we can support your next research or diagnostic endeavor.