Anti-IGFBP3 Rabbit Polyclonal Antibody for WB and ELISA - P17936

Core Product Specifications and Parameters

Biological Background: IGFBP3 Function and Localization

• IGFBP3 is a multifunctional protein that regulates the bioavailability of insulin-like growth factors (IGFs), thereby modulating IGF-mediated cell proliferation, differentiation, and apoptosis in a cell-type-specific manner (PubMed:10874028, PubMed:19556345). Related references: PMID:10874028 PMID:19556345
• It also exerts IGF-independent antiproliferative and apoptotic effects by binding to its receptor TMEM219/IGFBP-3R (PubMed:20353938) and via the LRP-1/alpha2M receptor, also known as TGF-beta type V receptor (PubMed:9252371). Related references: PMID:20353938 PMID:9252371
• IGFBP3 inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253) and promotes testicular germ cell apoptosis (PubMed:19952275). Related references: PMID:19623253 PMID:19952275
• Upon receptor engagement, IGFBP3 triggers serine-specific dephosphorylation of IRS1 or IRS2, initiating an inhibitory signaling cascade (PubMed:15371331). Related references: PMID:15371331
• In the nucleus, IGFBP3 interacts with transcription factors such as retinoid X receptor-alpha (RXRA) to regulate transcriptional signaling and apoptosis (PubMed:10874028). Related references: PMID:10874028
• The protein is secreted but also localizes to the nucleus, consistent with its dual role in extracellular signaling and intracellular transcriptional regulation.
• IGFBP3 is widely expressed in various tissues and is present in plasma; it undergoes glycosylation, phosphorylation, and disulfide bond formation.
• Alternative splicing of the IGFBP3 gene produces multiple isoforms, potentially contributing to its functional diversity.

Experimental Guidance and Technical Tips

• For Western blot, a primary band around 32 kDa is expected. Due to potential disulfide bonds and post-translational modifications, reduced and non-reduced conditions may yield different migration patterns; consider testing both.
• The antibody was raised against a peptide immunogen (aa 100-200); it may preferentially detect denatured protein, making it suitable for WB and ELISA but possibly requiring optimization for applications that require native conformation.
• Cross-reactivity with mouse and rat has been indicated; validate the antibody in lysates from these species to confirm recognition of the endogenous protein.
• For ELISA applications, pairing with a suitable capture antibody or using it as a detection antibody may be explored, and titration is recommended to determine optimal working concentrations.

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