Anti-IGFBP2 Rabbit Polyclonal Antibody for WB and ELISA - P18065

Core Product Specifications and Parameters

Biological Background: IGFBP2 Function and Localization

• Insulin-like growth factor-binding protein 2 (IGFBP2; aliases IBP2, IGF-BP53) is a secreted multifunctional protein that modulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner.
  Related references: PMID:18563800 PMID:38796567
• It regulates IGF1 signaling by coordinating with receptor protein tyrosine phosphatase beta (PTPRB) and the IGF1 receptor, stimulating PTEN phosphorylation and subsequent AKT1 activation.
  Related references: PMID:22869525
• IGFBP2 promotes cell migration via interaction with integrin alpha5 (ITGA5) through its RGD motif, enhancing endothelial progenitor cell adhesion to endothelial cells.
  Related references: PMID:16569642 PMID:26076738
• Upon mitochondrial damage, IGFBP2 facilitates apoptosis of podocytes by interacting with ITGA5, leading to phosphorylation of focal adhesion kinase (FAK) and mitochondrial injury.
  Related references: PMID:38796567
• The protein localizes to the extracellular space and is secreted, consistent with its role in extracellular signaling.
• IGFBP2 is a glycoprotein with disulfide bonds; its observed molecular weight (~35 kDa) may differ from the calculated 34.8 kDa due to post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 100-200 of human IGFBP2 (NP_000588.2), a region conserved in human, mouse, and rat, which supports the observed cross-reactivity.
• For Western blot (WB), expect a band at approximately 35 kDa; consider using a 10-12% SDS-PAGE gel and standard transfer conditions.
• For ELISA, the polyclonal nature may allow use as both capture and detection antibody, but optimal pairing should be validated in the relevant sample system.
• Due to glycosylation, the observed molecular weight may vary slightly; include appropriate molecular weight markers and consider de-glycosylation controls if needed.

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