Anti-IGFBP2 Monoclonal Antibody for WB, IHC-P, ELISA - P18065

Core Product Specifications and Parameters

Biological Background: IGFBP2 Function and Localization

• IGFBP2 is a multifunctional protein that regulates the availability of insulin-like growth factors (IGF1 and IGF2), modulating IGF-mediated processes such as cell proliferation, differentiation, and apoptosis in a cell-type specific manner. Related references: PMID:18563800, PMID:38796567

• It coordinates with receptor protein tyrosine phosphatase beta (PTPRB) and the IGF1 receptor to stimulate PTEN phosphorylation, leading to AKT1 activation. Related references: PMID:22869525

• IGFBP2 promotes cell migration via interaction with integrin alpha5 (ITGA5) through an RGD motif. Related references: PMID:16569642

• Interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Related references: PMID:26076738

• Upon mitochondrial damage, IGFBP2 facilitates apoptosis with ITGA5 of podocytes, activating FAK phosphorylation and mitochondrial injury. Related references: PMID:38796567

• IGFBP2 is a secreted glycoprotein with a signal peptide and disulfide bonds, characterized by growth factor binding and growth regulation functions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 226-325 of human IGFBP2; consider that this epitope is present in the full-length protein and may detect processed forms accordingly.
• For Western blot, the predicted molecular weight is approximately 35 kDa; include appropriate positive controls (e.g., human cell lines expressing IGFBP2) and optimize primary antibody dilution.
• In IHC-P, antigen retrieval conditions and antibody concentration should be optimized using tissues known to express IGFBP2 (e.g., liver, adipose tissue).
• For ELISA, this monoclonal antibody can be used as a capture or detection antibody; pairing with another clone recognizing a distinct epitope may enhance assay sensitivity.
• Since IGFBP2 is a secreted protein, consider analyzing cell culture supernatants or serum samples in addition to cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated monoclonal antibodies like the IGFBP2 Rabbit mAb for studying growth factor signaling and cancer biology. Our portfolio encompasses optimized antibody pairs, ancillary reagents, and rare-target sourcing to streamline your assay development and manufacturing.