Anti-IGFBP1 Rabbit Polyclonal Antibody for WB, ELISA - P08833

Core Product Specifications and Parameters

Biological Background: IGFBP1 Function and Localization

• Insulin-like growth factor-binding protein 1 (IGFBP1), also designated placental protein 12 (PP12), is a secreted multifunctional protein encoded by the IGFBP1 gene.
• It regulates the bioavailability of IGFs (IGF1 and IGF2) by binding them, thereby modulating IGF-mediated cellular processes including migration, proliferation, differentiation, and apoptosis in a cell-type specific manner.
  Related references:
  PMID:11397844
  PMID:15972819
• Through its RGD motif, IGFBP1 interacts with integrin ITGA5:ITGB1 to promote cell migration, activating focal adhesion kinase/PTK2 and the MAPK signaling pathway.
  Related references:
  PMID:7504269
  PMID:11397844
• In cardiomyocytes, IGFBP1 suppresses apoptosis by preventing HIF-1α/HIF1A ubiquitination and degradation (By similarity).
• It is a secreted phosphoprotein with a molecular mass of approximately 27.9 kDa, involved in growth factor binding.
• Keywords for this protein include secreted, phosphoprotein, growth factor binding, and reference proteome.

Experimental Guidance and Technical Tips

• Based on the immunogen design (recombinant protein corresponding to amino acids 150-250 of human IGFBP1), this polyclonal antibody is expected to recognize an internal region of the protein.
• For Western blot (WB), using a reducing condition may yield a band around 28 kDa; however, endogenous phosphorylation or glycosylation could cause subtle shifts—validate in the relevant sample system.
• In ELISA applications, consider using the antibody for capture or detection, but pairing with another clone should be validated.
• Given the secreted nature of IGFBP1, serum or plasma samples may require appropriate dilution to avoid matrix effects.
• Cross-reactivity is confirmed for human; reactivity with other species is not guaranteed and should be empirically tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare-target material procurement. This IGFBP1 polyclonal antibody supports research into IGF signaling, cell migration, and apoptosis—backed by CamelBio’s comprehensive technical and supply chain expertise.