Anti-IGFBP1 Monoclonal Antibody for WB, ELISA - P08833

Core Product Specifications and Parameters

Biological Background: IGFBP1 Function and Localization

• Insulin-like growth factor-binding protein 1 (IGFBP1), also known as placental protein 12 (PP12), is a secreted multifunctional protein that modulates the bioavailability of IGF1 and IGF2, thereby regulating cell migration, proliferation, differentiation, and apoptosis in a cell-type-specific manner. Related references: PMID:11397844 PMID:15972819
• Through its RGD motif, IGFBP1 binds integrin ITGA5:ITGB1, promoting cell migration by activating focal adhesion kinase (FAK) and the mitogen-activated protein kinase (MAPK) pathway. Related references: PMID:7504269
• IGFBP1 is highly expressed in the placenta and liver and is classified as a secreted protein following cleavage of its N-terminal signal peptide.
• Post‑translational modifications include phosphorylation and disulfide bond formation, which may influence its binding affinity and functional activity.
• The protein has a predicted molecular weight of approximately 28 kDa, consistent with the 27.9 kDa calculated from its amino acid sequence.
• Structural studies (3D-structure) and direct protein sequencing have confirmed its primary sequence and folding, providing a basis for epitope mapping and antibody development.

Experimental Guidance and Technical Tips

• The immunogen (aa 160–259) lies in a central region of human IGFBP1; this epitope may recognize both unmodified and post‑translationally modified forms.
• For Western blot analysis, the expected band migrates near 28 kDa; validate antibody specificity using positive and negative cell lysates.
• For ELISA, consider paired antibody formats (e.g., sandwich) and titrate concentrations to achieve optimal signal-to-noise ratios in your sample matrix.
• As the antibody is recommended for human samples, cross-reactivity with other species should be tested if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material supply solutions, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this anti‑IGFBP1 monoclonal antibody for growth factor and metabolic disorder research. Our capabilities include validated monoclonal/polyclonal antibodies, optimized matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to streamline your transition from early-stage assay development to reliable diagnostic application.