Anti-IGFALS Polyclonal Antibody for WB, ELISA - P35858

Core Product Specifications and Parameters

Biological Background: IGFALS Function and Localization

• IGFALS (Insulin-like growth factor-binding protein complex acid labile subunit) is a secreted protein predominantly found in plasma, where it forms ternary complexes with IGF-binding proteins (IGFBPs) and insulin-like growth factors (IGFs).
• The protein plays a critical role in protein-protein interactions, contributing to the formation of high molecular weight complexes that modulate IGF bioavailability and stability in circulation.
• Structurally, IGFALS contains leucine-rich repeats and multiple disulfide bonds, domains characteristic of proteins involved in protein binding and cell adhesion.
• Alternative splicing generates multiple isoforms, and the protein undergoes glycosylation, which may affect its stability and function.
• Subcellular localization is extracellular, consistent with its function as a carrier protein and its role in receptor-ligand binding.
• Genetic variations in IGFALS are associated with disease phenotypes, and the protein is widely detected in proteomics studies, underscoring its biological and clinical relevance.
• Additional annotations include direct protein sequencing evidence and a signal peptide, confirming its secretion pathway.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 396-605, NP_004961.1) corresponds to the C-terminal region of human IGFALS. For antibody validation, consider peptide competition assays using this recombinant protein fragment.
• In Western blot applications, expect a band at approximately 66 kDa under standard reducing conditions. Optimize sample preparation from plasma or cell lines expressing IGFALS; include appropriate loading controls.
• For ELISA, the antibody may be used to detect secreted IGFALS in biological fluids such as human plasma or cell culture supernatants. Validate cross-reactivity with mouse/rat samples if working with these species, and establish standard curves with recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, bulk reagents, and rare-target procurement to support diagnostic development from concept to clinic. This anti-IGFALS polyclonal antibody is part of our expanding catalog for growth factor biology and protein complex research, empowering reliable detection in immunoassays. For custom projects or technical consulting, our integrated supply chain ensures seamless access to critical raw materials and expertise.