Anti-IGF2BP3/IMP3 Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - O00425

Core Product Specifications and Parameters

Biological Background: IGF2BP3/IMP3 Function and Localization

• Encoded by the IGF2BP3 gene (aliases IMP3, KOC1, VICKZ3), is a member of the insulin-like growth factor 2 mRNA-binding protein family that contains KH and RRM RNA-binding domains.
• Functions as an RNA-binding factor that recruits target transcripts into cytoplasmic messenger ribonucleoprotein (mRNP) complexes, enabling mRNA transport, transient storage, and protection from endonucleolytic cleavage and microRNA-mediated decay.
• Preferentially binds to N6-methyladenosine (m6A)-modified mRNAs, enhancing their stability and translation regulation (PubMed:29476152). Related references: PMID:29476152
• Binds to and stabilizes CD44 and ACTB (beta-actin) transcripts; CD44 stabilization enhances cell adhesion and invadopodia formation in cancer cells.
• Binds to the coding region instability determinant (CRD) of MYC mRNA, increasing its stability; binding is enhanced by m6A modification of the CRD (PubMed:29476152). Related references: PMID:29476152
• Localizes to the nucleus, cytoplasm, P-bodies, and stress granules, reflecting dynamic roles in mRNA trafficking, decay, and stress response.
• Expressed in fetal tissues (liver, lung, kidney, thymus, placenta) and adult testis, ovary, brain, placenta; overexpressed in cervical, testicular, pancreatic, and renal carcinomas.
• Subject to phosphorylation and ubiquitin-like conjugation (e.g., SUMOylation), suggesting regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 300–579 of human IGF2BP3, a region predicted to be well-conserved across human, mouse, and rat, supporting the antibody's cross-reactivity.
• For Western blot (WB), a band of approximately 64 kDa is expected; however, post-translational modifications or isoforms may result in additional bands. Validate with positive and negative controls.
• In immunoprecipitation (IP), the rabbit monoclonal format provides high affinity; titrate the antibody amount to optimize pull-down efficiency while minimizing background.
• For immunofluorescence on paraffin-embedded sections (IF-P) or immunohistochemistry (IHC-P), standard antigen retrieval and permeabilization protocols are recommended. Test both cytoplasmic and nuclear staining patterns, as IGF2BP3 can localize to both compartments.
• The antibody is suitable for ELISA; consider using it as a capture or detection reagent in sandwich assays, paired with a validated detection antibody.
• Since IGF2BP3 is overexpressed in many cancers, include appropriate positive control tissues or cell lines (e.g., pancreatic cancer, cervical adenocarcinoma) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive catalog of IVD raw materials, including this recombinant rabbit monoclonal antibody against IGF2BP3/IMP3. For diagnostic developers and researchers focusing on cancer biomarkers and RNA-binding proteins, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare targets to support your assay development from concept to clinic.