Anti-IGF2BP2/IMP2 Rabbit Polyclonal Antibody for WB, IF, ELISA - Q9Y6M1

Core Product Specifications and Parameters

Biological Background: IGF2BP2/IMP2 Function and Localization

• Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2/IMP2) is an RNA-binding protein that belongs to the VICKZ family, also known as hepatocellular carcinoma autoantigen p62.
• Functions as a key regulator of mRNA fate by recruiting target transcripts into cytoplasmic mRNPs, enabling their transport, transient storage, and shielding from endonuclease cleavage or microRNA-mediated degradation (By similarity).
• Preferentially binds to N6-methyladenosine (m6A)-modified mRNAs, enhancing transcript stability. Related references: PMID:29476152
• Binds to the 5'-UTR of IGF2 mRNAs in an isoform-specific manner, modulating insulin-like growth factor 2 expression. Related references: PMID:9891060
• Stabilizes MYC mRNA by interacting with the coding region instability determinant (CRD); this interaction is strengthened by m6A modification within the CRD, promoting oncogenic signaling.
• Localizes to the nucleus and cytoplasm, including P-bodies and stress granules, reflecting dynamic shuttling and involvement in RNA processing and stress response.
• Expressed in a wide range of tissues, notably in reproductive organs and certain fetal tissues, with elevated levels observed in testicular cancer, hinting at roles in development and oncogenesis.
• Post-translational modifications such as acetylation and phosphorylation regulate its activity, and alternative splicing generates distinct isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 330–599, a region within the middle portion of the protein, which may contribute to good specificity for the target.
• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines known to express IGF2BP2/IMP2. Optimal dilution should be determined empirically.
• In immunofluorescence (IF) and immunofluorescence on paraffin sections (IF-P), appropriate fixation and permeabilization methods should be validated to preserve antigenicity.
• For ELISA, pairing with a suitable detection antibody may be necessary; users should check compatibility with their specific assay format.
• As a polyclonal antibody, it may recognize multiple isoforms; confirm specificity by using appropriate controls (siRNA knockdown, knockout lysates, etc.) where possible.

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