Anti-IGF2BP2/IMP2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6M1

Core Product Specifications and Parameters

Biological Background: IGF2BP2/IMP2 Function and Localization

• Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), also known as IMP2, p62, or VICKZ family member 2, is an RNA-binding protein that plays critical roles in mRNA transport, localization, and stability.
• IGF2BP2 recruits target transcripts into cytoplasmic messenger ribonucleoprotein (mRNP) complexes, enabling mRNA transport and transient storage, and protects them from endonucleolytic cleavage and microRNA-mediated degradation.
• IGF2BP2 preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and enhances their stability, contributing to post-transcriptional gene regulation. Related references: PMID:29476152
• Specifically binds to the 5'-untranslated region (5'-UTR) of IGF2 mRNAs in an isoform-specific manner, regulating expression of this growth factor. Related references: PMID:9891060
• IGF2BP2 binds to the coding region instability determinant (CRD) of MYC mRNA, stabilizing the transcript; this interaction is augmented by m6A modification of the CRD. Related references: PMID:29476152
• Subcellular localization includes the nucleus, cytoplasm, and cytoplasmic foci such as P-bodies and stress granules, reflecting its roles in mRNA trafficking and translational control.
• Tissue expression is observed in oocytes, granulosa cells, Leydig cells, spermatogonia, and various tissues (heart, placenta, skeletal muscle, etc.); notably, elevated protein levels are detected in testicular cancer.
• Post-translational modifications include acetylation and phosphorylation, and the protein is subject to alternative splicing and alternative translation initiation, which may generate functional diversity.

Experimental Guidance and Technical Tips

• For Western blot, the calculated molecular weight is 66 kDa; use appropriate protein markers and consider using whole cell lysates from HeLa or HEK293 cells as positive controls, as IGF2BP2 is ubiquitously expressed.
• In immunohistochemistry (IHC-P), perform antigen retrieval using heat-mediated citrate buffer (pH 6.0) and test on formalin-fixed paraffin-embedded human or mouse tissues known to express IGF2BP2, such as testis or ovary.
• For immunofluorescence/immunocytochemistry (IF/ICC), fix cells with 4% paraformaldehyde and permeabilize; validate localization patterns (cytoplasmic and nuclear) in relevant cell lines.
• The peptide immunogen lies in the C-terminal region (aa 500‑599), ensuring specificity; for ELISA, use the peptide as a positive control to confirm antibody reactivity.
• Since cross-reactivity is confirmed with mouse, this antibody can be used in murine models; however, validate in the specific strain and tissue of interest.
• Always include proper controls (isotype controls, blocking peptides) and optimize antibody dilutions for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-IGF2BP2, and ancillary reagents for mRNA regulation studies. With expertise in rare target sourcing and bulk supply, CamelBio streamlines procurement from concept to clinical application. Explore our portfolio for reliable RNA-binding protein research tools and custom manufacturing services.