Anti-IGF2BP2/IMP2 Polyclonal Antibody for IF/ICC, ELISA - Q9Y6M1

Core Product Specifications and Parameters

Biological Background: IGF2BP2/IMP2 Function and Localization

• Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), also known as IMP2 or VICKZ2, is a member of the VICKZ family of RNA-binding proteins that regulate mRNA fate.
• IGF2BP2 binds to target mRNAs and recruits them into cytoplasmic mRNP complexes, facilitating mRNA transport, transient storage, and protection from endonuclease cleavage and microRNA-mediated degradation (By similarity).
• It preferentially binds N6-methyladenosine (m6A)-modified mRNAs, enhancing their stability. This m6A-dependent stabilization is critical for key transcripts like MYC, where binding to the coding region instability determinant (CRD) is increased by m6A modification. Related references: PMID:29476152
• IGF2BP2 specifically interacts with the 5'-UTR of insulin-like growth factor 2 (IGF2) mRNAs in an isoform-specific manner, contributing to IGF2 translational control. Related references: PMID:9891060
• The protein localizes to the nucleus and cytoplasm, and can be found in cytoplasmic foci such as P-bodies and stress granules, consistent with its roles in mRNA storage and translational regulation.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its RNA-binding activity and localization.
• IGF2BP2 exhibits tissue-specific expression: it is detected in oocytes, granulosa cells, Leydig cells, spermatogonia, and semen, as well as in testicular cancer. It is weakly expressed in heart, placenta, skeletal muscle, and other tissues, and is found in fetal liver and fetal ovary.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC), consider optimizing permeabilization conditions to access both cytoplasmic and nuclear IGF2BP2 pools. Include positive (e.g., HeLa, HepG2) and negative control cells to confirm specificity.
• For ELISA, evaluate the antibody's performance with your target antigen under standard direct or indirect ELISA formats; pairing with a matched detection antibody may improve sensitivity.
• Because this antibody cross-reacts with mouse and rat IGF2BP2, validate reactivity in the relevant species' samples before large-scale experiments.
• As a polyclonal antibody, minor lot-to-lot variation is possible; always titrate and validate in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents. In addition to this anti-IGF2BP2/IMP2 polyclonal antibody, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for RNA-binding protein research. Our platform streamlines procurement of rare-target raw materials, helping you move efficiently from concept to clinic.