Anti-IGF2BP2/IMP2 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9Y6M1

Core Product Specifications and Parameters

Biological Background: IGF2BP2/IMP2 Function and Localization

• Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2, also known as IMP2, VICKZ2, and hepatocellular carcinoma autoantigen p62) is an RNA-binding protein encoded by the IGF2BP2 gene. Multiple isoforms exist due to alternative splicing and alternative initiation.
• IGF2BP2 recruits target transcripts into cytoplasmic messenger ribonucleoprotein (mRNP) complexes, enabling mRNA transport, transient storage, and translational regulation while shielding them from endonucleolytic cleavage and microRNA-mediated degradation.
• The protein preferentially binds N6-methyladenosine (m6A)-modified mRNAs, enhancing their stability. It stabilizes MYC mRNA by binding to its coding region instability determinant (CRD), with binding efficiency increased by m6A modification. Related references: PMID:29476152
• IGF2BP2 binds to the 5'-UTR of insulin-like growth factor 2 (IGF2) mRNA in an isoform-specific manner, and also interacts with ACTB (β-actin) mRNA. Related references: PMID:9891060
• Subcellularly, IGF2BP2 localizes to the nucleus, cytoplasm, P-bodies, and stress granules, reflecting its dynamic role in mRNA metabolism.
• Tissue expression is prominent in oocytes, ovarian granulosa cells, Leydig cells, spermatogonia, and testicular cancer. It is also detected at lower levels in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary gland, testis, and pancreas, as well as in fetal liver and ovary.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and subcellular distribution. The protein contains KH-type RNA-binding domains and is classified under RNA-binding, translation regulation, and mRNA transport pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–595, a region likely to be accessible in both the 66 kDa and 62 kDa isoforms. Consider this when selecting lysate or tissue types for detection.
• For Western blotting, expect bands near 66 kDa and 62 kDa; validate with appropriate positive controls (e.g., HeLa, HepG2, or testis-derived lines).
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution in tissues with known IGF2BP2 expression, such as testis, ovary, or certain carcinomas.
• For immunoprecipitation (IP) and ELISA, the monoclonal nature ensures batch-to-batch consistency, but always confirm performance in the relevant sample matrix.
• Cross-reactivity is reported only for human; for non-human samples, verify sequence homology and conduct a pilot experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, offering a comprehensive range of validated monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents. For researchers and diagnostic developers focusing on RNA-binding proteins like IGF2BP2/IMP2—an important marker in cancer and RNA biology—we provide reliable sourcing of antibody pairs and rare-target raw materials. Whether you need bulk quantities or support from concept to clinic, CamelBio bridges your supply chain with rigorous quality and technical expertise.