Anti-IGF2BP1/IMP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NZI8

Core Product Specifications and Parameters

Biological Background: IGF2BP1/IMP1 Function and Localization

• IGF2BP1 (Insulin-like growth factor 2 mRNA-binding protein 1), also known as IMP1, ZBP1, CRD-BP, and VICKZ1, is an RNA-binding protein that recruits target transcripts into cytoplasmic mRNPs to control their transport, storage, and translation.
• It acts as an N6-methyladenosine (m6A) reader, preferentially binding m6A-modified mRNAs and increasing their stability. This is exemplified by its interaction with the MYC mRNA coding region instability determinant (CRD), where m6A modification enhances binding, shielding the transcript from endonucleolytic cleavage and microRNA-mediated decay. Related references: PMID:29476152 PMID:32245947
• A well-characterized function is the transport and localized translation of ACTB (β-actin) mRNA: IGF2BP1 co-transcriptionally binds the 54-nucleotide zipcode element in the ACTB 3′-UTR; the RNP is then exported and transported along the cytoskeleton to the cell periphery. Phosphorylation of IGF2BP1 at the destination releases the mRNA for local actin polymerization, a process critical for cell migration and polarity.
• In neurons, IGF2BP1 regulates neurite outgrowth, growth cone guidance, and neuronal migration by controlling the spatial and temporal synthesis of proteins such as β-actin, and it may participate in mRNA trafficking to activated synapses.
• The protein shuttles between the nucleus and cytoplasm and localizes to perinuclear regions, P-bodies, stress granules, and various cell projections including lamellipodia, dendrites, growth cones, filopodia, and axons.
• IGF2BP1 is predominantly expressed in embryonic tissues (fetal liver, lung, kidney, thymus) and germ cells, and is frequently upregulated in cancers, consistent with its roles in promoting RNA stability and cell motility.

Experimental Guidance and Technical Tips

• For western blotting, expect a band around 63 kDa; include positive control lysates from human, mouse, or rat tissues/cell lines known to express IGF2BP1 (e.g., embryonic samples or cancer cells).
• In immunohistochemistry (IHC‑P), antigen retrieval may be necessary; validate staining patterns in tissues with documented expression, such as fetal liver or tumour specimens.
• Because IGF2BP1 is regulated by phosphorylation, check whether the epitope (aa 478–577) might be influenced by post‑translational modifications in your sample.
• For immunofluorescence/immunocytochemistry (IF/ICC), anticipate cytoplasmic staining with possible nuclear or cell‑projection signals; use appropriate fixation and permeabilization protocols.
• As a polyclonal antibody, lot‑to‑lot consistency can vary; re‑validate each new batch in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a streamlined, end‑to‑end sourcing solution for diagnostic and research raw materials. The IGF2BP1/IMP1 Rabbit pAb is part of our portfolio supporting cancer biology, neurobiology, and RNA metabolism studies. From validated antibody pairs for IVD kit development to bulk ancillary reagents and assistance in sourcing rare‑target raw materials, our platform consistently provides high‑quality products backed by expert consultation.