Anti-IGF2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01344

Core Product Specifications and Parameters

Biological Background: Insulin-like Growth Factor 2 (IGF2) Function and Localization

• Insulin-like growth factor 2 (IGF2), also known as IGF-II, Somatomedin-A, and T3M-11-derived growth factor, is encoded by the IGF2 gene. Alternative splicing generates multiple isoforms.
• Functions as a major fetal growth hormone, essential for fetoplacental development and tissue differentiation (By similarity). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle, and liver (Probable).
• Acts as a ligand for integrin, which is required for IGF2 signaling (PubMed:28873464). Positively regulates MYOD1 transcriptional coactivator recruitment, controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism by increasing mitochondrial respiration (By similarity). Related references: PMID:28873464
• The preptin peptide, derived from the E-domain of pro-IGF2, undergoes glucose-mediated co-secretion with insulin and amplifies glucose-mediated insulin secretion; exhibits osteogenic properties by promoting osteoblast mitogenic activity via MAPK1/3 (By similarity).
• Subcellular localization: Secreted protein (By similarity).
• Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen spans the propeptide region (aa 25–180) of human IGF2. The resulting polyclonal antibody may recognize both pro-IGF2 and mature IGF-II, as well as potential isoforms generated by alternative splicing.
• For Western blot (WB) analysis, the predicted molecular weight of the preproprotein is ~20 kDa (NP_000603.1). However, the mature secreted IGF-II peptide (~7.5 kDa) may appear as a lower band. Consider using reducing conditions and appropriate controls.
• Immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC) can benefit from tissues with high IGF2 expression, such as placenta, liver, muscle, and pancreas. Perform appropriate antigen retrieval and validate in the relevant sample system.
• ELISA applications can be developed using this antibody as a capture or detection reagent; however, optimal pairings with complementary antibodies or recombinant protein standards should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on fetal growth, metabolism, and tissue differentiation involving IGF2, we offer one-stop sourcing support, from concept to clinic, facilitating rare-target raw-material procurement and technical consulting.