Anti-IGF2 Rabbit Polyclonal Antibody for IF/ICC - P01344

Core Product Specifications and Parameters

Biological Background: IGF2 Function and Localization

• Insulin-like growth factor 2 (IGF2), also known as somatomedin-A, is a secreted growth factor belonging to the insulin family. It is produced as a preproprotein that undergoes proteolytic processing to generate the mature IGF2 and the Preptin peptide.

• IGF2 is a major fetal growth hormone, playing a critical role in fetoplacental development and tissue differentiation. It is influenced by placental lactogen.

• In adults, IGF2 is involved in glucose metabolism in adipose tissue, skeletal muscle, and liver. It also modulates muscle differentiation by positively regulating MYOD1 function and increasing mitochondrial respiration.

• IGF2 acts as a ligand for integrin, which is required for IGF2 signaling. Related references: PMID:28873464

• Preptin, a peptide derived from the IGF2 precursor, is co-secreted with insulin and amplifies glucose-mediated insulin secretion. It also exhibits osteogenic properties by enhancing osteoblast proliferation via MAPK1/3 activation.

• IGF2 is expressed in multiple tissues including heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye, and pancreas.

• The protein is subject to various post-translational modifications: it is glycosylated, contains disulfide bonds, and is cleaved at paired basic residues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 70-180 of human IGF2 (UniProt P01344). This region is conserved across human, mouse, and rat, consistent with the observed cross-reactivity. Researchers may consider verifying reactivity in other species.

• This antibody is intended for immunofluorescence/immunocytochemistry (IF/ICC). For Western blot or other applications, in-house validation is recommended.

• The predicted molecular weight of the unprocessed precursor is approximately 20 kDa, while the mature form migrates at around 26 kDa due to glycosylation and processing. Users should anticipate multiple bands depending on the sample and preparation conditions.

• Optimal working dilutions should be determined empirically for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This IGF2 antibody supports research into growth factor signaling, metabolic disorders, and developmental biology. For bulk orders or custom sourcing of rare targets, our team is ready to assist.