Anti-IGF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05019

Core Product Specifications and Parameters

Biological Background: IGF1 Function and Localization

• Insulin-like growth factor 1 (IGF1), also known as somatomedin-C or mechano growth factor (MGF), is a secreted protein structurally related to insulin and part of the insulin-like growth factor family.
• IGF1 exhibits potent growth-promoting activities, stimulating glucose transport, glycogen synthesis, and DNA synthesis in bone-derived osteoblastic cells, operating at lower concentrations than insulin.
• In the nervous system, IGF1 plays a role in synapse maturation, contributing to sensory perception such as smell in the olfactory bulb. Related references: PMID:21076856 PMID:24132240
• As a ligand for the IGF1 receptor (IGF1R), IGF1 binding activates the receptor's tyrosine kinase activity, triggering downstream PI3K-AKT/PKB and Ras-MAPK signaling cascades, and it also interacts with integrins ITGAV:ITGB3 and ITGA6:ITGB4 to modulate signaling. Related references: PMID:19578119 PMID:22351760 PMID:23243309
• Through the MAPK/ERK pathway, IGF1 acts as a negative regulator of apoptosis in cardiomyocytes, promoting the degradation of pro-apoptotic CREM isoforms.
• The IGF1 protein contains disulfide bonds essential for its structure and undergoes alternative splicing, resulting in multiple isoforms with distinct functions.
• Mutations in the IGF1 gene are associated with growth deficiencies, including dwarfism, as well as deafness and intellectual disability, highlighting its critical roles in development and organ function.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide spanning amino acids 50-150 of human IGF1 (NP_000609.1). For detection of endogenous IGF1, consider the protein's processing and secretion; the mature form may require optimization in sample preparation.
• For western blotting, a band around 22 kDa is expected for the pro-form or under non-reducing conditions; due to alternative splicing and post-translational modifications, multiple bands may occur. Validate in relevant positive control lysates (e.g., human liver or osteoblast cell lines).
• For immunohistochemistry (IHC-P), antigen retrieval methods and appropriate tissue fixation should be optimized, as IGF1 is secreted and may be localized to extracellular spaces.
• In ELISA applications, pair this antibody with a matched detection antibody for sandwich ELISA formats, or use as a capture/detection antibody depending on experimental design. Verify cross-reactivity with mouse and rat samples as indicated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering one-stop access to high-quality antibodies, antigens, and ancillary reagents for diagnostic development. Researchers focusing on growth factor signaling, metabolism, or neurobiology can leverage CamelBio's supply capabilities, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether you need technical consultation or procurement support from concept to clinic, CamelBio streamlines your workflow for this IGF1 antibody and beyond.