Anti-IFT88 Monoclonal Antibody for WB, ELISA - Q13099

Core Product Specifications and Parameters

Biological Background: IFT88 Function and Localization

• IFT88 (Intraflagellar transport protein 88 homolog) is a 93 kDa protein that positively regulates primary cilium biogenesis (PubMed:17604723). Related references: PMID:17604723
• It is also required for autophagy, specifically for trafficking of ATG16L and expansion of the autophagic compartment.
• The protein contains TPR repeats (tetratricopeptide repeats) and belongs to the TTC10 family, with alternative splicing producing multiple isoforms.
• IFT88 localizes to the centrosome, centriole, cilium basal body, and axoneme of primary cilia and flagella in various cell types.
• It is widely expressed in tissues including heart, brain, liver, lung, kidney, skeletal muscle, and pancreas.
• IFT88 is listed in proteomics identification databases and is a reference proteome component, highlighting its conserved role.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 534-833), which may detect multiple isoforms if this region is conserved.
• For Western blot, the predicted molecular weight is 93 kDa; actual migration may vary due to post-translational modifications or isoform differences.
• Consider optimizing antibody dilution for each application; typical starting dilutions for WB could be 1:500–1:2000, but require empirical determination.
• Cross-reactivity with mouse and rat has been reported; validate in the relevant species using appropriate controls.
• For ELISA, ensure coating and detection conditions are optimized for accurate quantification in cell lysates or recombinant proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic manufacturers and research laboratories. The IFT88 antibody supports investigations into ciliopathies, autophagy, and cell signaling, and CamelBio can assist with larger quantities, customized conjugations, or complementary reagents to accelerate your project from concept to clinic.